2384

Creatine Phosphokinase, Rabbit Skeletal Muscle

• Sinonimo/i: Creatine Phosphokinase, Rabbit Skeletal Muscle, rCPK
• Numero CAS: 9001-15-4
• Classificazione EC (Enzyme Commission): 2.7.3.2 (BRENDA, IUBMB)
• Numero MDL: MFCD00130876
• Codice UNSPSC: 12352202
• NACRES: NA.21

Proprietà

• Origine biologica: rabbit skeletal muscle
• Livello qualitativo: 200
• Stato: lyophilized
• Attività specifica: ≥300 U/mg, 25 °C; 750 U/mg, 37 °C
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; desiccated (hygroscopic)
• Solubilità: 0.1 M imidazole buffer, pH 6.6: soluble; 0.5% NaHCO₃, pH 9.0: soluble
• Attività estranea: ATPase, hexokinase, myokinase <0.001%
• Condizioni di spedizione: ambient
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Native creatine phosphokinase from rabbit muscle. Catalyzes the reversible conversion of creatine and ATP to creatine phosphate and ADP. Has an optimal pH of 9.0 for the forward reaction and 6.0-7.0 for the reverse reaction.

Attenzione

Toxicity: Standard Handling (A)

Definizione di unità

One unit is defined as the amount of enzyme that will catalyze the conversion of 1.0 µmol creatine phosphate per min at 25°C or 37°C, pH 6.8.

Ricostituzione

Following reconstitution in 0.5% NaHCO₃, refrigerate (4°C). Do not freeze or shake, as SH groups may be oxidized and form linkages between protein molecules, resulting in precipitation, turbidity, and loss of activity. NaHCO₃ stock solutions are stable for 2-3 days at 4°C. Following reconstitution in 0.1 M imidazole buffer, aliquot and freeze (-20°C). Imidazole stock solutions are stable for up to 4 weeks at -20°C. Avoid freeze/thaw cycles, as 20-30% of activity will be lost with each freeze/thaw cycle.

Altre note

Bessman, S.P. 1987. Anal. Biochem.161, 519.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable