235400

Caspase-3 Substrate I, Colorimetric

Colorimetric substrate for caspase-3 (Km = 9.7 µM) and related cysteine proteases.

• Sinonimo/i: Caspase-3 Substrate I, Colorimetric, Ac-DEVD- pNA, Ac-DEVD-pNA
• Formula empirica (notazione di Hill): C₂₆H₃₄N₆O₁₃
• Numero CAS: 1177131-27-9
• Peso molecolare: 638.58
• Codice UNSPSC: 12352200

Proprietà

• Saggio: ≥95% (HPLC)
• Forma fisica: lyophilized
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; desiccated; protect from light
• Solubilità: DMSO: 60 mg/mL
• Condizioni di spedizione: ambient
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Colorimetric substrate for CPP32/Apopain/Yama (K_m = 9.7 µM) and related cysteine proteases. Sequence is based on the P1-P4 tetrapeptide cleavage site of poly (ADP-ribose) polymerase (PARP) and includes Asp²¹⁶. CPP32 is the protease responsible for the cleavage of PARP. Can be used with Pro-caspase-3 in in vitro assays to measure caspase-9 activity, since caspase-9 activates pro-caspase-3. Cleavage of the substrate can be monitored at 405 nm (ε = 9160 cm^-1M^-1).

Colorimetric substrate for caspase-3 (K_m = 9.7 µM) and related cysteine proteases. Sequence is based on the P₁-P₄ tetrapeptide cleavage site of poly(ADP-ribose) polymerase (PARP) and includes Asp²¹⁶. Caspase-3 is the protease responsible for the cleavage of PARP. Also a substrate for caspase-6, caspase-7, caspase-8, and caspase-10. Cleavage of pNA is monitored colorimetrically at ~405 nm.

Azioni biochim/fisiol

Cell permeable: no

Km = 9.7 µM as colorimetric substrate for caspase-3

Primary Target
Caspas-3

Product does not compete with ATP.

Reversible: no

Attenzione

Toxicity: Standard Handling (A)

Sequenza

Ac-Asp-Glu-Val-Asp-pNA

Ricostituzione

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Altre note

Cardone, M.H., et al. 1998. Science 282, 1318.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Nicholson, D.W., et al. 1995. Nature376, 37.
Lazebnik, Y.A., et al. 1994. Nature371, 346.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable