233153-M

Cleland′s Reagent, ULTROL^® Grade

A protective agent for SH groups Absorbance: (100 mM, 280 nm):≤0.05. Oxidized dithiothreitol:≤0.5%.

• Sinonimo/i: Dithiothreitol, DTT
• Formula empirica (notazione di Hill): C₄H₁₀O₂S₂
• Numero CAS: 3483-12-3
• Peso molecolare: 154.25
• Numero MDL: MFCD00004877

Proprietà

• Livello qualitativo: 100
• Saggio: ≥99% (titration)
• Stato: solid
• Impiego in reazioni chimiche: reagent type: reductant
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze
• Colore: white
• Solubilità: water: 200 mM; ethanol: soluble
• Cationi in tracce: heavy metals: <1 ppm
• Temperatura di conservazione: 2-8°C
• InChI: 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m0/s1; VHJLVAABSRFDPM-IMJSIDKUSA-N

Descrizione

Descrizione generale

A protective agent for SH groups.

Absorbance: (100 mM, 280 nm):≤0.1. Oxidized dithiothreitol:≤0.5%.

DTT is a water-soluble protective reagent for sulfhydryl groups. It maintains monothiols in the reduced state and reduces disulfides quantitatively. Most widely used for the study of disulfide exchange reactions of protein disulfides. DTT readily permeates cell membranes, protecting protein sulfhydryls and restoring enzyme activity lost by the oxidation of sulfhydryl groups in vitro. As a reducing agent, it cyclizes as it reduces disulfides to thiols, so the reaction is "driven" to completion without using large excesses of reductant. If free thiols are to be assayed later, excess reductant must be removed by dialysis or some other appropriate method. Alternatively, an immobilized form of DTT (REDUCTACRYL Reagent, Cat. No. 233157) can be used. Absorbance: (100 mM, 280 nm):≤0.05. Oxidized dithiothreitol:≤0.5%.

Attenzione

Toxicity: Harmful (C)

Ricostituzione

Following reconstitution, aliquot and freeze (-20°C) under an inert gas. Avoid contact with metals. Stock solutions are stable for up to 3 months at -20°C.

Altre note

Chau, M-H., and Nelson, J.W. 1991. FEBS Lett.291, 296.
Lauriault, V.V.M., and O′Brien, P.J. 1991. Mol. Pharmacol.40, 125.
Klonne, D.R., and Johnson, D.R. 1988. Toxicol. Lett.42, 199.
Zhang, R., and Snyder, G.H. 1988. Biochemistry27, 3785.
Shaked, Z., et al. 1980. Biochemistry19, 4156.
Zahler, W.L., and Cleland, W.W. 1968. J. Biol. Chem.243, 716.
Cleland, W.W. 1964. Biochemistry3, 480.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

ULTROL is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Pittogrammi: GHS05,GHS07
• Avvertenze: Danger
• Indicazioni di pericolo: H302 - H315 - H318
• Consigli di prudenza: P264 - P270 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Classi di pericolo: Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable