219343

Cathepsin S, Human, Recombinant, E. coli

Cathepsin S, Human, Recombinant, E. coli, is prepared without a tag or fusion protein. A major lysosomal cysteine proteinase with high specific activity.

• Sinonimo/i: Cathepsin S Protein
• Classificazione EC (Enzyme Commission): 3.4.22.27 (BRENDA, IUBMB)
• Codice UNSPSC: 12352202
• NACRES: NA.77

Proprietà

• Livello qualitativo: 100
• Origine biologica: human
• Ricombinante: expressed in E. coli
• Saggio: ≥90% (SDS-PAGE)
• Forma fisica: liquid
• Attività specifica: ≥30,000 mU/mg protein
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; avoid repeated freeze/thaw cycles
• tecniche: cell based assay: suitable
• N° accesso ID proteina: AAC37592; AAH02642
• N° accesso UniProt: P25774
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −70°C
• Informazioni sul gene: human ... CTSS(1520)

Descrizione

Descrizione generale

Note: 1 mU = 1 milliunit.

Research area: Cell Signaling

Recombinant, human cathepsin S expressed in E. coli without a tag or fusion protein. A major lysosomal cysteine proteinase that is preferentially expressed in macrophages and microglia. Shown to be involved in the processing of antigenic peptides for presentation by MHC Class II molecules on the surface of antigen-presenting cells by mediating invariant (Ii) chain degradation. Inhibition of cathepsin S results in impaired antigen-presentation.

Applicazioni

Cathepsin S, Human, Recombinant, E. coli has been used in cathepsin digestion reactions.

Azioni biochim/fisiol

Cathepsin S is a cysteine protease that plays a role in degradation of class II complexes in human B lymphocytes. It possesses endoproteolytic activity and aids in processing and presenting antigens to immune cells, thereby influencing the immune response. Cathepsin S is involved in facilitating the degradation of damaged or unwanted proteins within the endo-lysosomal pathway. Dysregulation of Cathepsin S activity leads to the development of various diseases such as arthritis, cancer, and cardiovascular diseases.

Attenzione

Toxicity: Harmful (C)

Definizione di unità

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-VVR-AMC per min at 37°C, pH 6.5. Note: 1 mU = 1 milliunit.

Stato fisico

In 35 mM potassium phosphate, 35 mM Sodium acetate, 2 mM DTT, 2 mM EDTA, 50% ethylene glycol, pH 6.5.

Ricostituzione

Following initial thaw, aliquot and freeze (-70°C).

Altre note

Liuzzo, J.P., et al. 1999. Mol. Med.5, 334.
Riese, R.J., et al. 1998. J. Clin. Invest.101, 2351.
Sukhova, G.K., et al. 1998. J. Clin. Invest.102, 576.
Kirschke, H., and Wiederanders, B. 1994. Methods Enzymol.244, 500.
Xin, X.Q., et al. 1992. Arch. Biochem. Biophys.299, 334.
Kirschke, H., et al. 1989. Biochem. J.264, 467.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Pittogrammi: GHS08,GHS07
• Avvertenze: Warning
• Indicazioni di pericolo: H302,H373
• Consigli di prudenza: P260 - P264 - P270 - P301 + P312 - P314 - P501
• Classi di pericolo: Acute Tox. 4 Oral - STOT RE 2 Oral
• Organi bersaglio: Kidney
• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1