16-638
4G10® Platinum, Anti-Phosphotyrosine Antibody, Agarose Conjugate
clone 4G10®, Upstate®, from mouse
Sinonimo/i:
4G10 Platinum Anti-Phosphotyrosine, 4G10 Platinum Antibody, Phosphotyrosine Detection Antibody
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Tipo di anticorpo
primary antibodies
Clone
4G10®, monoclonal
Reattività contro le specie (prevista in base all’omologia)
all
Produttore/marchio commerciale
Upstate®
tecniche
immunoprecipitation (IP): suitable
Isotipo
IgG2bκ
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
phosphorylation (pTyr)
Informazioni sul gene
human ... PID1(55022)
Descrizione generale
The development of the anti-phosphotyrosine, clone 4G10 in 1989 was a monumental discovery for researchers. 4G10 was the first and best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10 is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. It has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 2 decades. Now Millipore has made the best even better with 4G10 Platinum. We pooled 4G10 with the next most highly regarded anti-phosphotyrosine, clone PY20, to make 4G10 Platinum. PY20 itself is a very poor substitute for 4G10, but its additive effect allow for a greater level of detection on more substrates that even 4G10 alone is capable.
Immunogeno
The immunogen for 4G10 was phospho-tyramine coupled to KLH, while the immunogen for PY20 was phosphotyrosine conjugated to a carrier protein.
Applicazioni
4G10 Platinum, Anti-Phosphotyrosine, Agarose Conjugate detects levels of Phosphotyrosine proteins & has been published & validated for use in IP.
Caratteristiche e vantaggi
Format: Gel Immobilized
Qualità
Immunoprecipitation:
Twenty μl of 50% slurry of this reagent (10 μl packed beads) was used to immunoprecipitate tyrosine-phosphorylated proteins from 250 μg PDGF-stimulated NIH3T3 cell lysate or EGF-stimulated A431 cell lysate.
Twenty μl of 50% slurry of this reagent (10 μl packed beads) was used to immunoprecipitate tyrosine-phosphorylated proteins from 250 μg PDGF-stimulated NIH3T3 cell lysate or EGF-stimulated A431 cell lysate.
Descrizione del bersaglio
Dependent upon the molecular weight of the Tyrosine-phosphorylated protein
Stato fisico
Supplied as a 50% slurry of a proprietary mixture of agarose conjugated, protein G purified mouse monoclonal 4G10 (IgG2bκ) and protein A purified PY20 (IgG2b) in PBS, pH 7.4 with 0.05% sodium azide.
protein G purified mouse monoclonal 4G10 (IgG2bκ) and protein A purified PY20 (IgG2b)
Stoccaggio e stabilità
Stable for 6 months at 2-8°C from date of shipment. For maximum recovery of product, centrifuge the vial prior to removing the cap.
NOTE: DO NOT FREEZE.
NOTE: DO NOT FREEZE.
Note legali
4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Certificati d'analisi (COA)
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
S Ruff-Jamison et al.
The Journal of biological chemistry, 266(10), 6607-6613 (1991-04-15)
Phosphotyrosine and similar analogs have been used to elicit antibodies that have found widespread use in the study of cellular tyrosine phosphorylation. In order to better understand the anti-phosphotyrosine immune response and to elucidate the details of the specific association
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