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Documenti fondamentali

05-321MG

Sigma-Aldrich

Anti-Phosphotyrosine Antibody, clone 4G10®

clone 4G10®, Upstate®, from mouse

Sinonimo/i:

Clone 4G10 Anti-Phosphotyrosine, Phosphotyrosine Detection Antibody

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

100

Forma dell’anticorpo

purified from hybridoma cell culture

Tipo di anticorpo

primary antibodies

Clone

4G10®, monoclonal

Reattività contro le specie (prevista in base all’omologia)

all

Produttore/marchio commerciale

Upstate®

tecniche

immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG2bκ

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

phosphorylation (pTyr)

Applicazioni

Anti-Phosphotyrosine Antibody, clone 4G10 detects level of Phosphotyrosine & has been published & validated for use in IP & WB.

Qualità

Routinely evaluated on EGF-treated human A431 carcinoma cells.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected tyrosine-phosphorylated proteins in a modified RIPA lysate from EGF-treated human A431 carcinoma cells (Cohen, B., 1990; Druker, B. J., 1989; Kanakura, Y., 1991).

Descrizione del bersaglio

Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.

Stato fisico

Format: Purified
Purified mouse monoclonal IgG2bκ in buffer containing PBS, pH 7.4. Liquid at 2-8ºC.
IgG2bκ mouse monoclonal antibody produced in vitro by mouse-mouse hybridoma 4G10 (FOX-NY [NS-1 derivative] myeloma x spleen cells).

Risultati analitici

Control
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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