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Key Documents

113200

Sigma-Aldrich

Anti-α-Actin Mouse mAb (1A4)

liquid, clone 1A4, Calbiochem®

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

Tipo di anticorpo

primary antibodies

Clone

1A4, monoclonal

Forma fisica

liquid

contiene

≤0.1% sodium azide as preservative

Reattività contro le specie

baboon, rabbit, human

Produttore/marchio commerciale

Calbiochem®

Condizioni di stoccaggio

do not freeze

Isotipo

IgG2a

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... ACTA1(58)

Descrizione generale

Mouse monoclonal antibody. Recognizes α-actin in smooth muscle cells.
Recognizes α-actin in smooth muscle cells of blood vessels and parenchymal tissue of the intestine, testis, and ovary. Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).
  • Antibody Target Gene Symbol: ACTA2
  • Target Synonym: 0610041G09RIK, a-SMA, AAT6, ACTSA, Actvs, ALPHA ACTIN VSM, ALPHA SMA, ALPHA SMOOTH MUSCLE ACTIN, ALPHA-ACTIN 2, FLJ45296, MGC127800, RGD: 621676, Similar to vascular alpha actin, Sm alpha actin, SMalphaA, SMOOTH MUSCLE ALPHA ACTIN
  • Entrez Gene Name: actin, alpha 2, smooth muscle, aorta
  • Hu Entrez ID: 59 (Related Antibodies: 113200)
  • Rat Entrez ID: 81633
  • This Anti-α-Actin Mouse mAb (1A4) is validated for use in Frozen Sections, Immunoblotting, Paraffin Sections for the detection of α-Actin.

    Immunogeno

    Human Smooth Muscle
    a synthetic peptide corresponding to amino acids 1-10 of human smooth muscle α-actin.

    Applicazioni

    Frozen Sections (1:50-1:75)

    Immunoblotting (see comments)

    Paraffin Sections (1:50-1:75)

    Confezionamento

    Please refer to vial label for lot-specific concentration.

    Attenzione

    Toxicity: Standard Handling (A)

    Stato fisico

    In 150 mM NaCl, 20 mM Tris-borate, BHC-6 carrier polysaccharides, pH 7.5.

    Risultati analitici

    Negative Control
    Fibroblasts, striated muscle, or myocardium
    Positive Control
    Smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, or ovary

    Altre note

    Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).Prolonged fixation in buffered formalin destroys the epitope. The antibody is specific for the α-smooth muscle isoform of actin. This antibody is also reported to work for immunoblotting. Variables associated with assay conditions will dictate the proper working dilution.
    Rannov-Jennsen, L., et al. 1990. Lab Invest.63, 532.
    Skalli, O., et al. 1989. J. Histochem. Cytochem37, 315.
    Skalli, O., et al. 1986. J. Cell. Biol. 103, 2787.

    Note legali

    CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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    Codice della classe di stoccaggio

    12 - Non Combustible Liquids

    Classe di pericolosità dell'acqua (WGK)

    WGK 2

    Punto d’infiammabilità (°F)

    Not applicable

    Punto d’infiammabilità (°C)

    Not applicable


    Certificati d'analisi (COA)

    Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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