07-896-I

Anti-phospho-Rac1/cdc42 (Ser71) Antibody

from rabbit, purified by affinity chromatography

• Sinonimo/i: Ras-related C3 botulinum toxin substrate 1, Cell migration-inducing gene 5 protein, Ras-like protein TC25, p21-Rac1, phospho-Rac1/cdc42 (Ser71)
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: human
• Reattività contro le specie (prevista in base all’omologia): rat (based on 100% sequence homology), zebrafish (based on 100% sequence homology), orangutan (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), Xenopus (based on 100% sequence homology), mouse (based on 100% sequence homology), chicken (based on 100% sequence homology)
• tecniche: immunoprecipitation (IP): suitable; western blot: suitable
• N° accesso NCBI: NP_008839
• N° accesso UniProt: P63000
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: phosphorylation (pSer71 )
• Informazioni sul gene: human ... RAC1(5879)

Descrizione

Descrizione generale

Target of rapamycin complex 2 subunit MAPKAP1 (UniProt Q9BPZ7; also known as MEKK2-interacting protein 1, Mitogen-activated protein kinase 2-associated protein 1, mSIN1, Ras inhibitor MGC2745, SAPK-interacting protein 1, Stress-activated protein kinase-interacting 1, Stress-activated map kinase-interacting protein 1,, TORC2 subunit MAPKAP1) is encoded by the MAPKAP1 (also known as JC310, MIP1 SIN1, SIN1b, SIN1g) gene (Gene ID 79109) in human. mSIN1 is an essential component of the Torc2 complex. mTORC2 is comprised of mTOR, a large Ser/Thr protein kinase, along with mSIN1, GbL (mLST8), Protor1, Protor2, and Rictor. The mTORC2 complex is activated primarily downstream of PI3 Kinase and is known to affect cell proliferation and survival primarily by phosphorylating Akt on Ser473. Additionally, the mTORC2 complex is known to affect cytoskeletal organization and cell migration by exerting its effects through Rac, Rho, and PKC. mSIN1 is also known to directly interact with Ras, SPAKs, and JNK.

Immunogeno

Epitope: Near N-Terminus

KLH-conjugated linear peptide corresponding to human phospho-Rac1/cdc42 (Ser71) near the N-Terminus.

Applicazioni

Detect Rac1/CDC42 using this rabbit Polyclonal Anti-phospho-Rac1/cdc42 (Ser71) Antibody, Cat. No. 07-896-I, validated for use in Western Blotting, Immunoprecipitation.

Immunoprecipitation Analysis: 2.5µg from a representative lot immunoprecipitated phospho-Rac1/cdc42 (Ser71) in EGF treated A431 cell lysate.

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

Qualità

Evaluated by Western Blotting in EGF treated A431 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho-Rac1/cdc42 (Ser71) in 10 µg of EGF treated A431 cell lysate.

Descrizione del bersaglio

~25 kDa observed

Stato fisico

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable