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Merck
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Documenti fondamentali

07-789

Sigma-Aldrich

Anti-phospho-AKT (Thr34) Antibody

Upstate®, from rabbit

Sinonimo/i:

Akt1, PKB, PRKBA, RAC, RAC-ALPHA

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

rabbit

Livello qualitativo

Forma dell’anticorpo

affinity purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Purificato mediante

affinity chromatography

Reattività contro le specie

human

Produttore/marchio commerciale

Upstate®

tecniche

western blot: suitable

Isotipo

IgG

N° accesso UniProt

Condizioni di spedizione

dry ice

modifica post-traduzionali bersaglio

phosphorylation (pThr34)

Informazioni sul gene

human ... AKT1(207)

Specificità

Predicted to cross react with Mouse and Rat due to high sequence homology, but have not been tested.
Recognizes phospho-AKT (Thr34)

Immunogeno

KLH conjugated peptide corresponding to amino acid region encompassing the human phospho-AKT (Thr34); Sequence highly conserved in mouse and rat.

Applicazioni

Anti-phospho-AKT (Thr34) Antibody detects level of phospho-AKT (Thr34) & has been published & validated for use in WB.
Research Category
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling

General Post-translation Modification

Qualità

Routinely evaluated by immunoblot.

Descrizione del bersaglio

60 kDa

Stato fisico

100 μL of epitope specific affinity purified rabbit polyclonal IgG in 50% affinity storage buffer (PBS containing 1.0 mg/mL BSA and 0.05% sodium azide) and 50% glycerol.
ImmunoAffinity Purified

Stoccaggio e stabilità

Stable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

Note legali

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2


Certificati d'analisi (COA)

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Cisplatin activates Akt in small cell lung cancer cells and attenuates apoptosis by survivin upregulation
Belyanskaya, Larisa L, et al
International Journal of Cancer. Journal International Du Cancer, 117, 755-763 (2005)
PKB/Akt: connecting phosphoinositide 3-kinase to cell survival and beyond.
Marte, B M and Downward, J
Trends in Biochemical Sciences, 22, 355-358 (1997)
Virginia Actis Dato et al.
Cells, 9(1) (2020-01-16)
Abstract: The cardiovascular disease (CVD) frequently developed during metabolic syndrome and type-2 diabetes mellitus is associated with increased levels of aggregation-prone small LDL particles. Aggregated LDL (aggLDL) internalization is mediated by low-density lipoprotein receptor-related protein-1 (LRP1) promoting intracellular cholesteryl ester
Xiao-Dong Fu et al.
PloS one, 3(7), e2790-e2790 (2008-07-31)
Progesterone plays a role in breast cancer development and progression but the effects on breast cancer cell movement or invasion have not been fully explored. In this study, we investigate the actions of natural progesterone and of the synthetic progestin
Virginia Actis Dato et al.
International journal of molecular sciences, 22(13) (2021-07-03)
Activated alpha-2 Macroglobulin (α2M*) is specifically recognized by the cluster I/II of LRP1 (Low-density lipoprotein Receptor-related Protein-1). LRP1 is a scaffold protein for insulin receptor involved in the insulin-induced glucose transporter type 4 (GLUT4) translocation to plasma membrane and glucose

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