07-582-I

Anti-phospho-IRF-3 (Ser396) Antibody

from rabbit, purified by affinity chromatography

• Sinonimo/i: Interferon regulatory factor 3, IRF-3
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: human
• Reattività contro le specie (prevista in base all’omologia): porcine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)
• tecniche: western blot: suitable
• N° accesso NCBI: NP_001562
• N° accesso UniProt: Q14653
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: phosphorylation (pSer396)
• Informazioni sul gene: human ... IRF3(3661) ; pig ... Irf3(396656) ; rhesus monkey ... Irf3(718970)

Descrizione

Descrizione generale

Interferon regulatory factors (IRFs) comprise a family of transcription factors that function with the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection. IRF-3 can inhibit cell growth and plays a critical role in controlling the expression of genes in the innate immune response. In unstimulated cells, IRF-3 is present in the cytoplasm. Viral infection results in phosphorylation of IRF-3 and leads to its translocation to the nucleus where it activates promoters containing IRF-3-binding sites. Phosphorylation of IRF-3 occurs at a cluster of C-terminal serine and threonine residues (between 385 and 405) leading to its association with the p300/CBP coactivator protein that promotes DNA binding and transcriptional activity.

Specificità

This antibody recognizes IRF-3 phosphorylated at Ser396.

Immunogeno

Epitope: SNS → ANA: Mutation of this residue results in loss of viral-induced phosphorylation of IRF-3.

KLH-conjugated linear peptide corresponding to human IRF-3 phosphorylated at Ser396.

Applicazioni

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Use Anti-phospho-IRF-3 (Ser396) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect phospho-IRF-3 (Ser396) also known as Interferon regulatory factor 3.

Qualità

Evaluated by Western Blot in untreated and sendai virus treated A549 cell lysates.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected phospho-IRF-3 (Ser396) in 10 µg of sendai virus treated A549 cell lysate.

Descrizione del bersaglio

~50 kDa observed. Uncharacterized bands may appear at ~40 kDa and ~80 kDa in some lysates.

Linkage

Replaces: 07-582

Stato fisico

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
Untreated and sendai virus treated A549 cell lysates

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable