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Documenti fondamentali

07-164

Sigma-Aldrich

Anti-phospho-H2A.X (Ser139) Antibody

Upstate®, from rabbit

Sinonimo/i:

H2A histone family, member Q, histone 2, H2ac, histone IIa, histone cluster 2, H2ac

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

rabbit

Livello qualitativo

100

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Reattività contro le specie

human, vertebrates

Confezionamento

antibody small pack of 25 μg

Produttore/marchio commerciale

Upstate®

tecniche

ChIP: suitable (ChIP-seq)
immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG

N° accesso NCBI

NP_002096

N° accesso UniProt

P16104

Condizioni di spedizione

ambient

modifica post-traduzionali bersaglio

phosphorylation (pSer139)

Informazioni sul gene

human ... HIST2H2AC(8338)

Descrizione generale

Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

Specificità

Broad species cross-reactivity expected based on conservation of sequence homology.
Histone H2A.X phosphorylated at Ser139.

Immunogeno

Epitope: a.a. 134-142

Applicazioni

Anti-phospho-H2A.X (Ser139) Antibody is a Rabbit Polyclonal Antibody for detection of phospho-H2A.X (Ser139) also known as histone 2 H2ac, histone IIa & has been validated in IP & WB.
Immunoprecipitation:
A previous lot of this antibody successfully immunoprecipitated phosphorylated H2A.X as determined by an independent laboratory.

Qualità

Routinely evaluated by Western Blot on untreated and staurosporine treated Jurkat lysate.

Western Blot Analysis:
1:500 dilution of this lot detected phospho-H2A.X on 10 μg of staurosporine treated Jurkat lysate.

Descrizione del bersaglio

15 kDa

Linkage

Replaces: MABE205

Stato fisico

Format: Purified

Stoccaggio e stabilità

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

Risultati analitici

Control
Staurosporine-treated Jurkat lysate, UV-treated HT-29 cells, doxorubicin-treated HT-29 cells.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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