07-408

Anti-Smad2/3 Antibody

Upstate^®, from rabbit

• Sinonimo/i: MAD, mothers against decapentaplegic homolog 2 (Drosophila), Mad protein homolog, Mad, mothers against decapentaplegic homolog 2, Mad-related protein 2, Mothers against DPP homolog 2, SMAD 2, SMAD family member 2, SMAD, mothers against DPP homolog 2, SMA
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: human, mouse, rat
• Confezionamento: antibody small pack of 25 μL
• Produttore/marchio commerciale: Upstate^®
• tecniche: western blot: suitable
• Isotipo: IgG
• N° accesso NCBI: NP_001003652
• N° accesso UniProt: Q15796
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... SMAD2(4087)

Descrizione

Descrizione generale

SMAD2 or Mothers against decapentaplegic homolog 2 is a polypeptide that, as its name describes, is a homolog of the Drosophila gene: "Mothers against decepentaplegic". It belongs to the SMAD family of proteins, which belong to the TGFbeta superfamily of modulators. Like many other TGFβ family members SMAD2 is involved in cell signalling. SMAD2 modulates signals of activin and TGFbeta′s. It interacts with SMAD anchor for receptor activation (SARA). The binding of ligands causes the phosphorylation of the SMAD2 protein and the dissociation from SARA and the association with SMAD4. It is subsequently transferred to the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD2 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.

Specificità

Recognizes human Smad2 Mr 55-60 kDa and Smad3 Mr 50 kDa as determined by using Smad2 and Smad3 transfected Cos cells.

Immunogeno

Fusion protein corresponding to amino acids 186-273 of human Smad2.

Applicazioni

Anti-Smad2/3 Antibody is a Rabbit Polyclonal Antibody for detection of Smad2/3 also known as MAD-mothers against decapentaplegic-homolog 2 & has been validated in WB.

Immunoblot Analysis: 1:1000-1:2000 dilution detected Smad2/3 in RIPA lysates of HepG2 cells.

Optimal working dilutiuons must be determined by end user.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Qualità

Routinely evaluated by Western Blot on Hela lysates.

Western Blot Analysis:
1:500 dilution of this lot detected SMAD2/3 on 10 μg of Hela lysates.

Descrizione del bersaglio

50-60 kDa

Linkage

Replaces: 04-1029

Stato fisico

ImmunoAffinity Purified

Purified rabbit polyclonal IgG in buffer containing 0.07 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol.

Stoccaggio e stabilità

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Risultati analitici

Control
HepG2 cell lysate.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.