07-1512

Anti-phospho-WAVE2 (Ser343) Antibody

from rabbit, purified by affinity chromatography

• Sinonimo/i: SCAR2, WASF2, dJ393P12.2, WASP family protein member 2, Protein WAVE-2, Verprolin homology domain-containing protein 2
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: human, monkey
• tecniche: western blot: suitable
• N° accesso NCBI: NP_008921
• N° accesso UniProt: Q9Y6W5
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: phosphorylation (pSer343)
• Informazioni sul gene: human ... WASF2(10163)

Descrizione

Descrizione generale

Wiskott-Aldrich Syndrome Protein (WASP)-family Protein Member 2 (known as WAVE2) is found in many cells and tissues, with strong expression in peripheral blood leukocytes. WAVE2 is involved in signaling RTKs and small GTPases. As a whole, the WAVE family of proteins have a general function for regulating the actin cytoskeleton in various tissues.

Specificità

This antibody recognizes WAVE2 phosphorylated at Ser343.

Immunogeno

Epitope: Phosphorylated Ser343

KLH-conjugated linear peptide corresponding to human WAVE2 phosphorylated at Ser343.

Applicazioni

Anti-phospho-WAVE2 (Ser343) Antibody detects level of phospho-WAVE2 (Ser343) & has been published & validated for use in WB.

Research Category
Cell Structure

Research Sub Category
Cytoskeletal Signaling

Western Blot Analysis: A previous lot of this antibody was used to detect endogenous phospho-WAVE2 (Ser343) in COS-7 and HMECs following EGF stimulation ± pretreatment with U0126 (Mendoza, M.C., et al. (2011). Mol Cell. 41(6):661-671).

Qualità

Evaluated by Western Blot in COS-7+EGF cell lysates untreated and lambda phosphatase-treated.

Western Blot Analysis: A 1:500 dilution of this antibody detected phospho-WAVE2 (Ser343) in 10 µg of COS-7+EGF cell lysates untreated and lambda phosphatase treated.

Descrizione del bersaglio

~75 kDa observed.
An uncharacterized band appears at ~103 kDa in some lysates.

Stato fisico

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
COS-7+EGF cell lysates untreated and lambda phosphatase treated.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable