06-519-AF488

Anti-phospho-CREB (Ser133) Antibody, Alexa Fluor^™ 488 conjugate

from rabbit, ALEXA FLUOR^™ 647

• Sinonimo/i: Cyclic AMP-responsive element-binding protein 1 (pSer133), cAMP-responsive element-binding protein 1 (pSer133), CREB-1 (pSer133), CREB-A (pSer133), htCREB (pSer133), CREB-B (pSer119)
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.45

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Coniugato: ALEXA FLUOR^™ 647
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Reattività contro le specie: human, rat, mouse
• Reattività contro le specie (prevista in base all’omologia): Xenopus (based on 100% sequence homology), bovine (based on 100% sequence homology), canine (based on 100% sequence homology), chicken (based on 100% sequence homology), porcine (based on 100% sequence homology), guinea pig (based on 100% sequence homology)
• tecniche: immunofluorescence: suitable
• Isotipo: IgG1
• N° accesso NCBI: NP_604391
• N° accesso UniProt: P16220
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: phosphorylation (pSer133)
• Informazioni sul gene: human ... CREB1(1385)

Descrizione

Descrizione generale

Cyclic AMP-responsive element-binding protein 1 (UniProt P16220; also known as cAMP-responsive element-binding protein 1, CREB-1) is encoded by the CREB1 gene (Gene ID 1385) in human. CREB is a transcription factor that binds the cAMP response elements (CRE) and regulates the transcription of the downstream genes. CREB activity is regulation via phosphorylation by various kinases, including PKA, and Ca2+/calmodulin-dependent protein kinases. Genes known to be regulated by CREB include c-fos, BDNF, tyrosine hydroxylase, neuropeptides (such as somatostatin, enkephalin, VGF, and corticotropin-releasing hormone), as well as the circadian clock genes PER1 and PER2. CREB is critical for memory formation across a wide range of species.

Specificità

Recognizes phosphorylated CREB-A (pSer133), htCREB (pSer133), and CREB-B (pSer119) spliced isoforms reported by (UniProt P16220). May also recognize phosphorylated CREM and ATF-1 that contain homologous sequence to that of the immungen peptide.

Immunogeno

Epitope: pSer133.

KLH-conjugated synthetic peptide corresponding to the amino acids including and encompassing phosphorylated Ser133 of CREB-A.

Applicazioni

Anti-phospho-CREB (Ser133) Antibody, Alexa Fluor^™ 488 conjugate is an antibody against phospho-CREB for use in Immunofluorescence.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

The unconjugated antibody (Cat. No. 06-519) is shown to be suitable also for Electrophoretic Mobility Shift Assay (EMSA), immunohistochemistry, and Western blotting applications.

Qualità

Evaluated by Immunofluorescence in rat brain tissue.

Immunofluorescence Analysis: A 1:50 dilution of this antibody detected phospho-CREB (Ser133) in rat brain tissue.

Descrizione del bersaglio

~43 kDa observed.

Stato fisico

Affinity purified

Purified rabbit polyclonal antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Note legali

ALEXA FLUOR is a trademark of Life Technologies

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable