05-503-I

Anti-LacI Antibody, clone 9A5

clone 9A5, from mouse

• Sinonimo/i: Lactose operon repressor
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 9A5, monoclonal
• Reattività contro le specie: E. coli
• tecniche: western blot: suitable
• Isotipo: IgG1κ
• N° accesso NCBI: NP_414879
• N° accesso UniProt: P03023
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: Escherichia coli K12 ... Laci(945007)

Descrizione

Descrizione generale

The lactose operon repressor binds to a regulatory element, an operator gene, to form a genetic switch, the lac operon. This switch negatively regulates the expression of structural genes that follow the site of the operator gene. Inducers may bind to the repressor to reduce or eliminate the affinity of the repressor for its operator, and thereby allow for transcription of respective structural genes. Conversely, other effector molecules may increase the binding affinity of the repressor for its operator and thereby inhibit gene transcription. The inducer or effector molecules that associate with a repressor serve as a chemical signal, which monitors the metabolic state of protein(s) expressed by their respective genes.

Immunogeno

TrpE fused with full-length E. Coli LacI.

Applicazioni

Anti-LacI Antibody, clone 9A5 is an antibody against LacI for use in Western Blotting.

Research Category
Secondary & Control Antibodies

Research Sub Category
Epitope Tags

Qualità

Evaluated by Western Blot in Rosetta BL21 (DE3) E. Coli cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected LacI in 10 µg of Rosetta BL21 (DE3) E. Coli cell lysate.

Descrizione del bersaglio

~38 kDa observed

Stato fisico

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide (This antibody is purified from supernatent.).

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
Rosetta BL21 (DE3) competent E. Coli cell lysate.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable