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05-457

Sigma-Aldrich

Anti-Histone H1 Antibody, clone AE-4

clone AE-4, Upstate®, from mouse

Sinonimo/i:

H1 histone family, member 4, Histone H1b, histone 1, H1e, histone cluster 1, H1e

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

AE-4, monoclonal

Reattività contro le specie

bovine, human, plant

Produttore/marchio commerciale

Upstate®

tecniche

ELISA: suitable
immunocytochemistry: suitable
western blot: suitable

Isotipo

IgG2a

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

dry ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

bovine ... H1-4(617854)
human ... H1-4(3008)

Descrizione generale

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

Specificità

Predicted to stain nuclei from other animal and plant species.
This antibody recognizes histone H1, Mr 32-33 kDa. Doublet detected due to hyperphosphorylation of histone H1 subpopulation. Cross-reacts with other histone isoforms in the following order of signal strength: H3, H2B > H4 > H2A, as determined with recombinant protein.

Immunogeno

Nuclei from myeloid leukemia biopsy cells. Clone AE-4.

Applicazioni

Immunocytochemistry:
5-20 µg/mL of a previous lot showed positive nuclear immunostaining in A431 cells fixed with 4% paraformaldehyde and methanol.

ELISA:
Recommended.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Use Anti-Histone H1 Antibody, clone AE-4 (mouse monoclonal antibody) validated in ELISA, ICC, WB to detect Histone H1 also known as H1 histone family member 4, Histone H1b, histone 1 H1e, histone cluster 1 H1e.

Qualità

Routinely evaluated by immunoblot on lysates from an acid extract of HeLa cells.

Western Blot:
1-4 µg/mL of this lot detected histone H1 in lysates from an acid extract of HeLa cells.

Descrizione del bersaglio

32-33 kDa (Histone H1); Doublet (hyperphosphorylated Histone H1); 17 kDa (other histone isoforms).

Stato fisico

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2a in buffer containing PBS, pH 7.2, with 0.1% sodium azide. Frozen solution.

Stoccaggio e stabilità

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Risultati analitici

Control
Nuclear extracts from NIH3T3 or HeLa cells.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Nicotine induces chromatin remodelling through decreases in the methyltransferases GLP, G9a, Setdb1 and levels of H3K9me2.
Chase, KA; Sharma, RP
The International Journal of Neuropsychopharmacology null
Jung Ha Choo et al.
BMC genomics, 8, 479-479 (2008-01-02)
MacroH2A1 is a histone variant that is closely associated with the repressed regions of chromosomes. A recent study revealed that this histone variant is highly enriched in the inactive alleles of Imprinting Control Regions (ICRs). The current study investigates the
Super-resolution microscopy reveals decondensed chromatin structure at transcription sites.
Wang, Y; Maharana, S; Wang, MD; Shivashankar, GV
Scientific Reports null
Characterisation of human histone H1x.
Nicole Happel, Ekkehard Schulze, Detlef Doenecke
Biological Chemistry null
Jae Hyun Lee et al.
Human molecular genetics, 18(14), 2567-2574 (2009-04-22)
We recently described two opposing states of transcriptional competency. One is termed 'competent' whereby a gene is capable of responding to trans-acting transcription factors of the cell, such that it is active if appropriate transcriptional activators are present, though it

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