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Documenti fondamentali

05-157

Sigma-Aldrich

Anti-MAPK1/ERK2 Antibody

Upstate, mouse monoclonal, 1B3B9

Sinonimo/i:

Extracellular signal-regulated kinase 2, MAP kinase 2, MAPK 2, Mitogen-activated protein kinase 2, extracellular signal-regulated kinase-2, mitogen-activated protein kinase 1, protein tyrosine kinase ERK2.

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Nome del prodotto

Anti-MAP Kinase 2/Erk2 Antibody, clone 1B3B9, clone 1B3B9, Upstate®, from mouse

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

1B3B9, monoclonal

Reattività contro le specie

avian, mouse, human, rat

Produttore/marchio commerciale

Upstate®

tecniche

immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG2a

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

dry ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

Descrizione generale

ERK2 is activated by MEK2, which phosphorylates neighboring threonine 183 and tyrosine 185 residues. ERK2 is a serine/threonine kinase that phosphorylates MAP2 and myelin basic protein. This kinase is an important proximal component of the MAP kinase pathway involved in transeitting the signals from growth factors, neurotransmitters and hormones at the cell surface to the transcriptional events in the nucleus.

Specificità

Other species cross reactivity is unknown.
This antibody recognizes MAP kinase encoded by the mapk gene, Mr 42 kDa.

Immunogeno

HPLC purified murine recombinant MAP Kinase. Clone 1B3B9

Applicazioni

Anti-MAP Kinase 2/Erk2 Antibody, clone 1B3B9 is an antibody against MAP Kinase 2/Erk2 for use in IP & WB.
Immunoprecipitation:
4 μg of a previous lot immunoprecipitated MAP Kinase 2/Erk2 from a RIPA lysate from mouse 3T3 cells. Boil the cell lysate and let cool before adding the antibody, as this antibody only binds to denatured MAP Kinase.
Research Category
Signaling
Research Sub Category
MAP Kinases

Qualità

Routinely evaluated in RIPA lysates from human A431 carcinoma cells, mouse 3T3 fibroblasts, or rat L6 cells.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected MAP Kinase 2/Erk2 in RIPA lysates from human A431 carcinoma cells. A previous lot detected MAP Kinase in mouse 3T3 fibroblasts and rat L6 cells.

Descrizione del bersaglio

42 kDa

Linkage

Replaces: 04-349

Stato fisico

Format: Purified
Protein G Chromatography
Purified mouse monoclonal IgG2a in buffer containing PBS with 0.05% sodium azide. Store frozen at -20°C, avoid freeze thaw cycles.

Stoccaggio e stabilità

Stable for 1 year at -20ºC from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Risultati analitici

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Mitogen-activated protein kinase modulates ethanol inhibition of cell adhesion mediated by the L1 neural cell adhesion molecule.
Dou, X; Wilkemeyer, MF; Menkari, CE; Parnell, SE; Sulik, KK; Charness, ME
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Allelic variation of the ACCase gene and response to ACCase-inhibiting herbicides in pinoxaden-resistant Lolium spp.
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Reversion of Ras- and phosphatidylcholine-hydrolyzing phospholipase C-mediated transformation of NIH 3T3 cells by a dominant interfering mutant of protein kinase C lambda is accompanied by the loss of constitutive nuclear mitogen-activated protein kinase/extracellular signal-regulated kinase activity.
Bjorkoy, G, et al.
The Journal of Biological Chemistry, 272, 11557-11565 (1997)
Cytosolic phospholipase A2 is phosphorylated in collagen- and thrombin-stimulated human platelets independent of protein kinase C and mitogen-activated protein kinase.
Borsch-Haubold, A G, et al.
The Journal of Biological Chemistry, 270, 25885-25892 (1995)
Y Ihara et al.
The Journal of biological chemistry, 272(15), 9629-9634 (1997-04-11)
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