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Key Documents

05-1511

Sigma-Aldrich

Anti-Y14 Antibody, clone 4C4

clone 4C4, from mouse

Sinonimo/i:

Binder of OVCA1-1, RNA binding motif protein 8A, RNA binding motif protein 8B, RNA-binding motif protein 8A, RNA-binding protein Y14, Ribonucleoprotein RBM8A, ribonucleoprotein RBM8

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

4C4, monoclonal

Reattività contro le specie

mouse, rat, human

tecniche

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG2bκ

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... RBM8A(9939)

Descrizione generale

Y14 (RNA binding motif protein 8A or RBM8A), an RNA-binding protein, contains a conserved RNA-binding motif. Y14 is found predominantly in the nucleus, although it is also present in the cytoplasm. It is preferentially associated with mRNAs produced by splicing, including both nuclear mRNAs and newly exported cytoplasmic mRNAs. It is thought that the protein remains associated with spliced mRNAs as a tag to indicate where introns had been present, thus coupling pre- and post-mRNA splicing events. Previously, it was thought that two genes encode this protein, RBM8A and RBM8B; it is now thought that the RBM8B locus is a pseudogene. Two alternative start codons result in two forms of the protein, and this gene also uses multiple polyadenylation sites.

Specificità

This antibody recognizes Y14.

Immunogeno

Epitope: Unknown
Purified human Y14.

Applicazioni

Anti-Y14 Antibody, clone 4C4 is a Mouse Monoclonal Antibody for detection of Y14 also known as Binder of OVCA1-1, RNA binding motif protein 8A, Ribonucleoprotein RBM8A & has been validated in WB, ICC & IP.
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Y14 in HeLa and NIH/3T3 cells.

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in immunoprecipitation. Kashima, I., et al. (2006). Genes & Dev. 20:355-367.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
RNA Binding Protein (RBP)

Qualità

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected Y14 on 10 µg of HeLa cell lysate.

Descrizione del bersaglio

24 kDa

Stato fisico

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
HeLa cell lysate

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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