05-1336-S
Anti-phospho-Histone H3 (Ser10) Antibody, clone CMA312, Trial Size
clone CMA312, Upstate®, from mouse
Sinonimo/i:
H3S10P, Histone H3 (phospho Ser10), H3 Histone, family 3A
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Forma dell’anticorpo
purified antibody
Tipo di anticorpo
primary antibodies
Clone
CMA312, monoclonal
Reattività contro le specie
human, vertebrates
Produttore/marchio commerciale
Upstate®
tecniche
ChIP: suitable
ELISA: suitable
immunocytochemistry: suitable
multiplexing: suitable
western blot: suitable
Isotipo
IgG1
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
phosphorylation (pSer10)
Informazioni sul gene
human ... H3C1(8350)
Descrizione generale
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming the nucleosome. Histones are modified post-translationally; and these modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. Phosphorylation at Ser10, which is linked to gene activation, prevents methylation at Lys9 but facilitates acetylation of H3 and H4. Phosphorylated Ser10 is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition, Ser10 phosphorylation is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at Ser10 by Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin.
Specificità
Broad species cross-reactivity is expected, based on sequence homology.
This antibody specifically recognizes Histone H3 phosphorylated at Ser10.
Immunogeno
Epitope: Phospho Ser10
Synthetic peptide corresponding to amino acids 1-19 of human Histone H3, phosphorylated on Ser10, conjugated to KLH.
Applicazioni
Anti-phospho-Histone H3 (Ser10) Antibody, clone CMA312 is a mouse monoclonal antibody for detection of phospho-Histone H3 (Ser10) also known as H3S10P, Histone H3 (phospho S10) & has been validated in WB, ELISA, ICC, Multiplexing and ChIP.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Histones
Chromatin Biology
Histones
Qualità
Western Blot Analysis: 1 μg/mL of this antibody detected phospho-Histone H3 (Ser10) on 1 μg of colcemid-treated HeLa acid extract.
Descrizione del bersaglio
~17 kDa
Stato fisico
Format: Purified
Protein G Chromatography
Purified mouse monoclonal IgG in PBS with 0.05% sodium azide.
Stoccaggio e stabilità
Stable for 1 year at 2-8°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at 2-8°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Risultati analitici
Control
HeLa Acid extract lysate
HeLa Acid extract lysate
Altre note
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Note legali
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Raccomandato
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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