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Documenti fondamentali

04-928

Sigma-Aldrich

Anti-Histone H3 Antibody, CT, pan, clone A3S, rabbit monoclonal

culture supernatant, clone A3S, Upstate®

Sinonimo/i:

H3, Histone H3

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
A3S, monoclonal
application:
ChIP
WB
Reattività contro le specie:
human, rat, Saccharomyces cerevisiae, mouse, chicken
tecniche:
ChIP: suitable
western blot: suitable
citations:
23

Origine biologica

rabbit

Livello qualitativo

Forma dell’anticorpo

culture supernatant

Tipo di anticorpo

primary antibodies

Clone

A3S, monoclonal

Reattività contro le specie

human, rat, Saccharomyces cerevisiae, mouse, chicken

Produttore/marchio commerciale

Upstate®

tecniche

ChIP: suitable
western blot: suitable

Isotipo

IgG

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

dry ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... H3F3B(3021)

Descrizione generale

An antibody that recognizes the C-terminus of histone H3 and is useful for identifying total H3 independant of post-translational modifications.

Specificità

Broad species cross-reactivity expected due to sequence homology
Recognizes Histone H3.

Immunogeno

KLH-conjugated, synthetic peptide corresponding to the C-terminus of human Histone H3

Applicazioni

Anti-Histone H3 Antibody, CT, pan, clone A3S is a Rabbit Monoclonal Antibody for detection of Histone H3 also known as Histone H3 & has been validated in ChIP & WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Western Blot Analysis: 1:500-1:2,000 dilution of this lot detected Histone H3 in a modification independent manner in acid extracted proteins from untreated, sodium butyrate or colcemid treated HeLa cells.

Chromatin Immunoprecipitation:
2 μL of of a previous lot of this antibody immunoprecipitated chromatin associated with Histone H3 from a wild type yeast lysate.

Qualità

Routinely evaluated by immunoblot analysis.

Descrizione del bersaglio

17 kDa

Linkage

Replaces: 05-928

Stato fisico

Cultured supernantant in 0.05% sodium azide

Stoccaggio e stabilità

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Note legali

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Marcel Donnet et al.
Journal of colloid and interface science, 340(2), 218-224 (2009-10-10)
Thermodynamic solubility calculations are normally only related to thermodynamic equilibria in solution. In this paper, we extend the use of such solubility calculations to help elucidate possible precipitation reaction pathways during the entire reaction. We also estimate the interfacial energy
Huiyuan Zhang et al.
Nature communications, 5, 5798-5798 (2014-12-17)
The transcriptional regulator STAT3 curbs pro-inflammatory cytokine production mediated by NF-κB signalling in innate immune cells, yet the mechanism by which this occurs has been unclear. Here we identify STAT3 as a pivotal negative regulator of Ubc13, an E2 ubiquitin-conjugating
Histone H3 lysine 4 methylation is mediated by Set1 and required for cell growth and rDNA silencing in Saccharomyces cerevisiae.
Briggs, S D, et al.
Genes & Development, 15, 3286-3295 (2001)
Jessica Kain et al.
Molecular metabolism, 53, 101291-101291 (2021-07-12)
Type II nuclear hormone receptors, including farnesoid X receptors (FXR), liver X receptors (LXR), and peroxisome proliferator-activated receptors (PPAR), which serve as drug targets for metabolic diseases, are permanently positioned in the nucleus and thought to be bound to DNA
Phosphorylation of RNA polymerase II CTD regulates H3 methylation in yeast.
Xiao, Tiaojiang, et al.
Genes & Development, 17, 654-663 (2003)

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