UFC9003
Filtri centrifughi Amicon® Ultra, MWCO 3 kDa
sample volume 15 mL, regenerated cellulose membrane, MWCO 3 kDa
Sinonimo/i:
Concentratore centrifugo con membrana Ultracel®, MWCO 3 kDa, volume del campione 15 mL
About This Item
Prodotti consigliati
Materiali
polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing
Livello qualitativo
Sterilità
non-sterile
Nome Commerciale
Amicon®
Produttore/marchio commerciale
Amicon® Ultra
Parametri
15 mL sample volume
tecniche
protein extraction: suitable
protein purification: suitable
Lunghezza
12.1 cm (4.807 in.)
Diametro
2.97 cm
Superficie filtrante
7.6 cm2
Volume
15 mL
Dimensione pori
3 kDa MWCO
Condizioni di spedizione
ambient
Descrizione generale
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. ®
Applicazioni
- ®
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
- Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS.
- Concentration of dilute or pre-purified proteins from column eluents.
- Desalting, buffer exchange and protein dialysis.
Caratteristiche e vantaggi
- ®
- Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes).
- Heat-sealed membrane minimizes downstream extractables.
- 100% integrity tested for reliable performance.
- Convenient sample monitoring with translucent housing and volume gradations.
- Direct pipettor sample access eliminates processing step to recover concentrate.
- High concentration factors of 80–100X.
Stato fisico
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Articoli
Filtration methods and membrane filter selection in nanoparticle purification and production.
This page discusses the principles and benefits of using ultrafiltration membranes in protein sample preparation. Possible applications include solvent exchange, protein sample concentration, or sample purification.
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