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packaging
pkg of 10 vials (5x200µL aliquots of each component)
concentration
≥5x108 VP/ml (via p24 Assay)
application(s)
CRISPR
shipped in
dry ice
storage temp.
−70°C
1 of 4
This Item | 17-704 | 17-700 | 17-10523 |
---|---|---|---|
Upstate® | Upstate® | Magna RIP®, Upstate® | - |
100 | 100 | 100 | 100 |
kit of 12 assay(s) | kit of 48 assay(s) | - | - |
General description
Custom pools for follow-up screening or 10x Genomics Compatible CRISPR pools are also available by contacting your local sales representative.
Application
- Functional Genomics/Target Validation
- Focused forward genetic activation screening
- Validated positive and negative controls
- Set up and optimization of screen assay
- Creation of cell lines stably expressing KRAB-dCas9
Features and Benefits
- Focus on your research, and we will generate your lentivirus screening library
- Compact and ready to use CRISPRi library ~6 sgRNAs
- Optimized (F+E) gRNA scaffold (pools are gRNA-only, KRAB-dCas9 sold separately)
- Can be paired with CRISPRICON to ease optimization using validated positive control targeting RAB1A and non-targeting control for assay setup
- UCOE KRAB-dCas9 for consistent effector expression across a wide variety of cell lines
- Ease of optimization: Utilizes BFP and Puromycin as selection markers under EF1alpha promoter.
Components
PCRISPR003A - Human CRISPR Inhibition Dolcetto Library Set A
PCRISPR003B - Human CRISPR Inhibition Dolcetto Library Set B
Principle
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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