221198

Formamide

ACS reagent, ≥99.5%

• Synonym(s): Amide C₁, Formic amide
• Linear Formula: HCONH₂
• CAS Number: 75-12-7
• Molecular Weight: 45.04
• Beilstein: 505995
• EC Number: 200-842-0
• MDL number: MFCD00007941
• UNSPSC Code: 12352111
• PubChem Substance ID: 24853220

Properties

• grade: ACS reagent
• Quality Level: 200
• vapor density: 1.55 (vs air)
• vapor pressure: 0.08 mmHg ( 20 °C); 30 mmHg ( 129 °C)
• Assay: ≥99.5%
• form: liquid
• autoignition temp.: 932 °F
• technique(s): PCR: suitable
• color: APHA: ≤10
• refractive index: n20/D 1.447 (lit.)
• pH: 4-10 (20 °C, 200 g/L)
• bp: 210 °C (lit.)
• mp: 2-3 °C (lit.)
• transition temp: freezing point 2.0-3.0 °C
• density: 1.134 g/mL at 25 °C (lit.)
• SMILES string: NC=O
• InChI: 1S/CH3NO/c2-1-3/h1H,(H2,2,3)
• InChI key: ZHNUHDYFZUAESO-UHFFFAOYSA-N

Description

Application

The addition of formamide may enhance the specificity and efficiency of amplification of PCR (polymerase chain reaction). It may be used as an ammonia equivalent for the palladium-catalyzed aminocarbonylation of 1-bromo-3-trifluoromethylbenzene to form 3-(trifluoromethyl)benzamide.

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H351 - H360D - H373
• Precautionary Statements: P201 - P202 - P260 - P280 - P308 + P313 - P405
• Hazard Classifications: Carc. 2 - Repr. 1B - STOT RE 2 Oral
• Target Organs: Blood
• Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 1
• Flash Point(F): 305.6 °F
• Flash Point(C): 152 °C
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves