G005723
LC/MS Analysis of Polypeptide Drugs on Ascentis® Express RP-Amide
application for HPLC
About This Item
technique(s)
HPLC: suitable
test parameters
column: Ascentis Express RP-Amide, 10 cm x 2.1 mm I.D., 2.7 μm particles (53913-U)
mobile phase: [A] 10 mM ammonium acetate in water, pH 4.2; [B] 10 mM ammonium acetate in 90:10 (v/v) acetonitrile:water, pH 4.7
gradient: held at 20% B for 0.1 min; 20 to 80% B in 2.9 min; 80 to 20% B in 0.01 min; held at 20% B for 0.99 min
flow rate: 0.3 mL/min
column temp.: 35 °C
detector: TOF, ESI(+), m/z 100-2500
injection: 3 μL
sample: 1 μL/mL in 10mM ammonium formate, 80:20, acetonitrile:water
suitability
application for HPLC
application(s)
life science and biopharma
Analysis Note
Legal Information
Analyte
mobile phase component
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