53913-U
Ascentis® Express 90 Å RP-Amide (2.7 μm) HPLC Columns
L × I.D. 10 cm × 2.1 mm, HPLC Column
Synonym(s):
Core-shell (SPP) Fused Core polar embedded HPLC column
About This Item
Recommended Products
product name
Ascentis® Express RP-Amide, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 10 cm × 2.1 mm
material
stainless steel column
Quality Level
Agency
suitable for USP L60
product line
Ascentis®
feature
endcapped
manufacturer/tradename
Ascentis®
packaging
1 ea of
parameter
60 °C temp. range
600 bar max. pressure (9000 psi)
technique(s)
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
L × I.D.
10 cm × 2.1 mm
surface area
135 m2/g
impurities
<5 ppm metals
matrix
Fused-Core® particle platform
superficially porous particle
matrix active group
amide, alkyl phase
particle size
2.7 μm
pore size
90 Å
operating pH range
2-9
application(s)
food and beverages
separation technique
reversed phase
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Legal Information
Application
guard cartridge
related product
required but not provided
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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A significantly improved HPLC-fluorescence method for DMB-NANA and -NGNA, and application of this method to compare 2 candidate biosimilar therapeutic proteins to their respective RMs.
Sigma-Aldrich.com presents an article about innovative particle technology and RP-Amide bonding chemistry provide the benefits of speed, resolution, selectivity, aqueous stability, and improved peak shape for bases.
Pistachios contain approximately 45% fat, which can result in a significant amount of coextracted matrix in the acetonitrile extract generated using the QuEChERS procedure. A zirconia based adsorbent significantly reduces coextracted matrix prior to LC-MS or GC-MS.
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Protocols
Separation of null
THC solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material; Cannabidiolic acid solution, 1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material; Cannabichromene solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material; Cannabigerolic acid solution, 1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material; Δ9-Tetrahydrocannabinolic acid A (THCA-A)
THC solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material; Cannabidiolic acid solution, 1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material; Cannabichromene solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material; Cannabigerolic acid solution, 1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material; Δ9-Tetrahydrocannabinolic acid A (THCA-A)
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Chromatograms
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