ISO2

Mouse Monoclonal Antibody Isotyping Reagents

sufficient for 1000 tests (clones) (by ELISA), sufficient for 40 tests (clones) (by immunodiffusion, ODD)

• Synonym(s): Mouse Antibody Isotyping Kit
• MDL number: MFCD08705607
• UNSPSC Code: 12352203
• NACRES: NA.32

Properties

• usage: sufficient for 1000 tests (clones) (by ELISA); sufficient for 40 tests (clones) (by immunodiffusion, ODD)
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

The Mouse Monoclonal Antibody Isotyping Reagents enable qualitative isotype determination of mouse monoclonal antibosies derived from hybridoma supernatant, ascites fluid or purified forms.

Specificity

ISO-2 contains 0.2 mL vials of subclass specific antibodies to all four mouse IgG subclasses, IgA, and IgM. Directions are included for using these reagents in ELISA and Ouchterlony assays to determine the subclass of monoclonal antibodies in ascites, culture supernatants, or purified preparations. Additional reagents may be required, such as conjugates for ELISA assays or agarose and buffer components for Ouchterlony assays.

Application

Mouse Monoclonal Antibody Isotyping Reagents has been used in monoclonal antibody isotype determination using enzyme-linked immunosorbent assay (ELISA). It may be used in Ouchterlony immunodiffusion assays.

Determination of the subclass of a monoclonal antibody is helpful for characterization of the antibody, for choosing detection reagents, and for deciding on a purification scheme.

Features and Benefits

• May be used in a variety of assay formats
• Suitable for all antibody forms
• Determines all mouse IgG subclasses, IgA, and IgM

Kit Components Only

• goat antisera to mouse IgA .2 mL
• goat antisera to mouse IgG3 .2 mL
• goat antisera to mouse IgG1 .2 mL
• goat antisera to mouse IgG2a .2 mL
• goat antisera to mouse IgG2b .2 mL
• goat antisera to mouse IgM .2 mL

Safety Information

• Storage Class Code: 10 - Combustible liquids