HeLa S3

87110901

• Synonym(s): HeLa-S3 Cells, HeLa/S3 Cells, HeLaS3 Cells, S3 HeLa Cells
• UNSPSC Code: 41106514

Properties

• biological source: human cervix
• growth mode: Suspension
• karyotype: Modal no. 68
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human cervix carcinoma

Cell Line Description

Derived from the parent HeLa cell line. The cells have been adapted to grow in suspension (spinner) culture and have the same virus susceptibility as the parent line. Very useful in the clonal analysis of mammalian cell populations relating to chromosomal variation, cell nutrition and plaque forming ability. The cells should be handled under laboratory containment level 2.

Application

HeLa S3 has been used to study the cytotoxicity of dinuclear titanium-salan complexes. It has also been used to perform in vitro genotoxicity tests (comet and micronucleus) to study the effects of occupational exposure to carcinogens on sewage workers.

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 14
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18

Culture Medium

SMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) +10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Culture in a spinner flask. This cell line may adhere if the culture is not agitated.

Other Notes

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