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Key Documents

HeLa Ohio

84121901

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About This Item

UNSPSC Code:
41106514

biological source

human cervix

growth mode

Adherent

karyotype

Aneuploid

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human cervix carcinoma

Cell Line Description

Derivative of parent HeLa line (Sigma catalogue no. 93021013).

Application

Tumourigenicity and virus studies: poliovirus 1 and Ad 3

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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