B1256

Bromocresol Green sodium salt

crystalline

• Synonym(s): 3′,3″,5′,5″-Tetrabromo-m-cresolsulfonphthalein sodium salt
• Empirical Formula (Hill Notation): C₂₁H₁₃Br₄NaO₅S
• CAS Number: 62625-32-5
• Molecular Weight: 720.00
• EC Number: 263-657-4
• MDL number: MFCD00148898
• UNSPSC Code: 12171500
• PubChem Substance ID: 24891573
• NACRES: NA.47

Properties

• Agency: suitable for SM 2310; suitable for SM 2320
• Quality Level: 200
• form: crystalline
• visual transition interval: 3.8-5.4, yellow to blue
• mp: 230 °C (dec.) (lit.)
• solubility: H₂O: 1 mg/mL
• λmax: 616 nm in H₂O
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].Cc1c(Br)c(O)c(Br)cc1\C(=C2\C=C(Br)C(=O)C(Br)=C2C)c3ccccc3S([O-])(=O)=O
• InChI: 1S/C21H14Br4O5S.Na/c1-9-12(7-14(22)19(26)17(9)24)21(13-8-15(23)20(27)18(25)10(13)2)11-5-3-4-6-16(11)31(28,29)30-21;/h3-8,26-27H,1-2H3;/q;+1/p-1
• InChI key: HEFSGAHJDGZCHA-UHFFFAOYSA-M

Description

Application

Bromocresol Green sodium salt has been used as a pH indicator for glutamate decarboxylase activity assay in Listeria monocytogenes strains.

Biochem/physiol Actions

Bromocresol Green is mainly used for measuring serum albumin. In presence of acidic conditions, bromocresol green interacts with albumin, thereby resulting in an increase in absorbance at 630nm. The change in absorbance is linked with the concentration of albumin. Bromocresol Green is also a pH indicator, changing from yellow (pH 4.0) to blue color (5.4).

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves