PPC1010
Protease and Phosphatase Inhibitor Cocktail
DMSO solution, for the inhibition of serine, cysteine, acid proteases, aminopeptidase, serine/threonine protein phosphatases and L-isozymes of alkaline phosphatase
Synonym(s):
Protease and Phosphatase Inhibitor Mix, Protease and phosphatase inhibitor
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352200
NACRES:
NA.54
form:
DMSO solution
storage temp.:
−20°C
Recommended Products
Product Name
Protease and Phosphatase Inhibitor Cocktail,
form
DMSO solution
Quality Level
packaging
pkg of 1 ml, 5 ml ×
shipped in
wet ice
storage temp.
−20°C
Related Categories
General description
Protease and Phosphatase Inhibitor Cocktail is designed to increase the yield of intact and unmodified proteins in cell extracts. Protease and Phosphatase Inhibitor cocktail mixture inhibits serine, cysteine, and acid proteases, as well as aminopeptidase. It also inhibits serine/threonine protein phosphatases and L-isozymes of alkaline phosphatase. The cocktail has been tested on extracts from various animal tissues such as the human placenta and bovine liver and on extracts of A431, CHO, and U937 cells. Protease and Phosphatase Inhibitor Cocktail is supplied as a ready-to-use solution in DMSO.
Application
Protease and Phosphatase Inhibitor Cocktail has been used as a supplement in radioimmunoprecipitation assay (RIPA) buffer:
- for cellular protein extraction
- to lyse purified CD4+ T cells
- to prepare protein extracts from placental tissues and cells
Protease and Phosphatase Inhibitor Cocktail has been used in protein isolation buffers.
Preparation Note
The recommended dilution of the cocktail in the biological extract is 1 ml of the cocktail per 500 mg of protein extracted from tissue or cells. In many cases, the cocktail can be used at a final concentration of 1% (v/v), 1 ml of cocktail solution per 100 ml of extraction buffer.
Other Notes
This product is for R&D use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
188.6 °F
Flash Point(C)
87 °C
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Marcela Rassi-Cruz et al.
Endocrine-related cancer, 28(1), 1-13 (2020-10-29)
Familial primary aldosteronism (PA) is rare and mostly diagnosed in early-onset hypertension (HT). However, 'sporadic' bilateral adrenal hyperplasia (BAH) is the most frequent cause of PA and remains without genetic etiology in most cases. Our aim was to investigate new
E Zoico et al.
Mechanisms of ageing and development, 192, 111374-111374 (2020-10-06)
Fibrosis has been considered as a hallmark of dysfunctional adipose tissue (AT), however the role and mechanisms of fibrosis in the age related AT dysfunction are not yet well characterized. The aim of the study was to investigate the mechanisms
Beta-Arrestin1 Prevents Preeclampsia by Downregulation of Mechanosensitive AT1-B2 Receptor Heteromers
Quitterer U, et al.
Cell, S0092-8674(18), 31406-31406 (2018)
Sylwia Dabrowska et al.
Experimental neurology, 336, 113531-113531 (2020-11-23)
Stroke is the leading cause of long-term, severe disability worldwide. Immediately after the stroke, endogenous inflammatory processes are upregulated, leading to the local neuroinflammation and the potentiation of brain tissue destruction. The innate immune response is triggered as early as
Graphene quantum dots prevent alpha-synucleinopathy in Parkinson?s disease
Kim D, et al.
Nature Nanotechnology, 13(9), 812-812 (2018)
Related Content
An overview of cell lysis and protein extraction methods including detergent solubilization, freeze-thaw lysis, osmotic shock, sonication, enzymatic cell lysis, and mechanical disruption techniques such as Dounce, Polytron, and mortar and pestle homogenization.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service