71296

PhosphoSafe Extraction Reagent

Novagen′s PhosphoSafe Extraction Buffer efficiently extracts cytosolic proteins from mammalian and insect cells while preserving their phosphorylation state.

• Synonym(s): Protein Extraction Reagent
• UNSPSC Code: 41106510
• NACRES: NA.77

Properties

• Quality Level: 100
• form: liquid
• manufacturer/tradename: Novagen^®
• storage condition: OK to freeze
• technique(s): immunoblotting: suitable
• application(s): sample preparation
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

PhosphoSafe Extraction Buffer efficiently extracts cytosolic proteins from mammalian and insect cells while preserving their phosphorylation state. This reagent contains the same formula as CytoBuster Protein Extraction Reagent (Trim 2001), but also includes four phosphatase inhibitors: sodium fluoride, sodium vanadate, β-glycerophosphate, and sodium pyrophosphate. PhosphoSafe Extraction Reagent is compatible with kinase assays, protein interaction analysis, and other applications. This reagent is provided in convenient 25-ml bottles (1 bottle for 71296-3, 5 bottles for 71296-4).

Features and Benefits

• Phosphorylation state preserved during extraction
• Compatible with kinase assays

Warning

Toxicity: Toxic (F)

Other Notes

Trim, J.E. and Sawyer, D.L. 2001. inNovations12, 5.

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable