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Key Documents

GE17-0489-01

Superose® 6 Prep Grade

Cytiva 17-0489-01, pack of 125 mL

Synonym(s):

Size exclusion chromatography, Superose 6 column

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About This Item

UNSPSC Code:
41115709
NACRES:
NA.56

packaging

pack of 125 mL

manufacturer/tradename

Cytiva 17-0489-01

matrix

highly cross-linked agarose

particle size

30 μm

cleaning in place

1-14

working range

3-12

General description

Good resolution separations of proteins and other biomolecules according to size, Wide separation range – separations in two molecular weight ranges from 1000 up to 5 million, Excellent reproducibility and durability.
Superose® 6 is designed for high-resolution chromatography. Superose® 6 prep grade permits easy, efficient scale-up to preparative separations. Superose® 12 is for high resolution purification of proteins from 1 × 103 to 3 × 105 molecular weight, while Superose® 12 prep grade is useful for preparative purification of these higher molecular weight proteins.

Features and Benefits

  • Achieve high-resolution separations across exceptionally broad molecular-weight ranges
  • Low ionic strength eluents (< 0.05 M) can utilize a hydrophobic interaction component to alter the selectivity of Superose® for some lipids, peptides, and small aromatic compounds
  • High performance gel filtration media specially designed for preparative purification of biomolecules

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Superose is a registered trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Setting Column Pressure Limits for Size Exclusion Chromatography

Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.

Protocols

Performing a Separation with Superose

Superose from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superose column.

Sample Preparation for Size Exclusion Chromatography

Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of the chromatographic medium.

Related Content

Separation Options with Superose®

Separation options for Size Exclusion Chromatography with Superose from Cytiva.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service