3T3 clone A31 Cell Line from mouse
86110401, mouse embryo, Fibroblast
Synonym(s):
3T3-A31 Cells, BALB/3T3 clone A31 Cells, BALB/c 3T3 clone A31 Cells
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About This Item
UNSPSC Code:
41106514
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product name
3T3 clone A31 Cell Line from mouse, 86110401
biological source
mouse embryo
description
Mouse BALB/c embryo
growth mode
Adherent
karyotype
2n = 40, hypotetraploid
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse BALB/c embryo
Cell Line Description
Cells have now lost their contact inhibition.
Application
3T3 clone A31 cell line from mouse has been used:
- to study in vitro cytotoxicity and genotoxicity of retinyl palmitate–loaded poly(lactide-co-glycolide) nanoparticles for the topical treatment of skin diseases
- for the characterization of the phototoxicity of Vemurafenib, which is a B-Raf serine/threonine kinase inhibitor
- for the phototoxicity assessment of systemically applied compounds and drug candidates
Tumourigenicity and virus studies: SV40 and MSV
Culture Medium
DMEM + 2mM Glutamine + 5% New Born Calf Serum (NBCS) + 5% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 5x1,000 to 2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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