1.12553

Coomassie Brilliant blue R 250 (C.I. 42660)

for electrophoresis Trademark of Imperial Chemical Industries PLC

• Synonym(s): Coomassie Brilliant blue R 250 (C.I. 42660), protein stain
• Empirical Formula (Hill Notation): C₄₅H₄₄N₃NaO₇S₂
• CAS Number: 6104-59-2
• Molecular Weight: 825.97
• MDL number: MFCD00041762
• UNSPSC Code: 41105305
• EC Index Number: 228-060-5

Properties

• Quality Level: 100
• potency: >5000 mg/kg LD₅₀, oral (Rat)
• technique(s): protein staining: suitable
• pH: 6.2 (25 °C, 10 g/L in H₂O)
• mp: 174-180 °C
• solubility: 20 g/L
• bulk density: 250 kg/m³
• εmax: ≥300 at 554-563 nm at 0.025 g/L (pH 7.0)
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-30°C
• InChI: 1S/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54;/h7-30,46H,4-6,31-32H2,1-3H3,(H-,49,50,51,52,53,54);/q;+1/p-1
• InChI key: HAEGGLCXLFWTBE-UHFFFAOYSA-M

Description

Analysis Note

Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ_max. (buffer pH 7.0): 554 - 563 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.025 g/l; buffer pH 7.0; calc. on dried substance): ≥ 300
TLC-Test: passes test
Loss on drying (110 °C): ≤ 5 %
Suitability for electrophoresis: passes test

Legal Information

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable