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B6916

Supelco

Bradford Reagent

for 0.1-1.4 mg/ml protein

Synonym(s):

Coomassie dye binding protein assay, Coomassie dye binding protein assay, Protein dye reagent, Protein dye reagent

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About This Item

UNSPSC Code:
12161500
NACRES:
NA.32

Quality Level

200

form

solution

storage temp.

2-8°C

General description

Bradford assay is addition of coomassie brilliant blue G-250 to protein solution. The coomassie blue dye associates with basic and aromatic amino acids, thereby causing shift in absorbance during protein determination.

Application

Bradford Reagent has been used to determine total protein concentration.

Features and Benefits

  • The reagent is ready to use. No mixing or dilution required.
  • Color development is rapid. Only a five minute incubation and then the sample is read a 595 nm.
  • Reducing sugars and reducing substances along with thiols do not interfere with this reagent.
  • Reagent is suitable for micro (1-10 μg/ml) and standard (50-1400 μg/ml) assays.
  • Can be used in microwell plate assays.
  • Inexpensive assay.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

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Pictograms

Health hazardCorrosion
GHS08,GHS05

Signal Word

Warning

Hazard Statements

H290,H315,H319,H371

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 2

Target Organs

Eyes,Central nervous system

Storage Class Code

8B - Non-combustible corrosive hazardous materials

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Rosenberg IM
Protein Analysis and Purification: Benchtop Techniques (2006)
Proteomic response of the biological control fungus Trichoderma atroviride to growth on the cell walls of Rhizoctonia solani.
Grinyer J et al.
Current Genetics, 47, 381-381 (2005)
Caleigh Mandel-Brehm et al.
Neurology, 93(5), e433-e444 (2019-07-05)
To identify molecular correlates of primary angiitis of the CNS (PACNS) through proteomic analysis of CSF from a biopsy-proven patient cohort. Using mass spectrometry, we quantitatively compared the CSF proteome of patients with biopsy-proven PACNS (n = 8) to CSF
Tracey Welham et al.
Journal of experimental botany, 60(12), 3353-3365 (2009-05-29)
Neutral/alkaline invertases are a subgroup, confined to plants and cyanobacteria, of a diverse family of enzymes. A family of seven closely-related genes, LjINV1-LjINV7, is described here and their expression in the model legume, Lotus japonicus, is examined. LjINV1 previously identified
Feng He et al.
BMC plant biology, 19(1), 552-552 (2019-12-14)
Understanding lignin biosynthesis and composition is of central importance for sustainable bioenergy and biomaterials production. Species of the genus Miscanthus have emerged as promising bioenergy crop due to their rapid growth and modest nutrient requirements. However, lignin polymerization in Miscanthus
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Protein Quantitation

Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.

Tools for Protein Quantitation

A wide variety of products for traditional protein quantitation techniques such as BCA and Bradford. Also, products for alternative assays such as Lowry, Micro Pyrogallol and FluoroProfile are also available for total protein determination.

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