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SRP3316

Sigma-Aldrich

LIF human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

Synonym(s):

D factor, Differentiation-stimulating factor, Melanoma-derived LPL inhibitor (MLPLI)

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.77
Pricing and availability is not currently available.

recombinant

expressed in E. coli

Assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

mol wt

19.6 kDa

impurities

endotoxin, tested

UniProt accession no.

P15018

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... LIF(3976)

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1 of 4

This Item
QBD10246QBD10127QBD11086
Bis-dPEG®13-NHS ester

QBD10954

Bis-dPEG®13-NHS ester

Bis-dPEG®9-NHS ester

QBD10246

Bis-dPEG®9-NHS ester

Acid-dPEG®13-NHS ester

QBD10127

Acid-dPEG®13-NHS ester

m-dPEG ®13-NHS ester

QBD11086

m-dPEG ®13-NHS ester

form

solid or viscous liquid

form

solid or viscous liquid

form

solid or viscous liquid

form

solid or viscous liquid

assay

>90%

assay

>90%

assay

>90%

assay

>90%

reaction suitability

reagent type: cross-linking reagent
reactivity: amine reactive

reaction suitability

reagent type: cross-linking reagent
reactivity: amine reactive

reaction suitability

reagent type: cross-linking reagent
reactivity: amine reactive

reaction suitability

reagent type: chemical modification reagent, reagent type: cross-linking reagent
reactivity: amine reactive

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

General description

Leukemia inhibitory factor (LIF) is a secreted pleiotropic glycoprotein which is a member of the gp130 signaling of interleukin-6 (IL-6) family cytokines.[1][2] This protein was originally identified as a protein that prevented the growth of a leukemic cell line.[3]
Recombinant human LIF is a 19.6 kDa protein containing 180 amino acids residues including three disulfide bonds.

Biochem/physiol Actions

Leukemia inhibitory factor (LIF) suppresses the differentiation of myoblasts, and inhibits the activation of caspase-3 and blocks the reduction in Bcl-xL (B-cell lymphoma-extra large) levels, thus, preventing doxorubicin-induced apoptosis. Along with transforming growth factor β2 (TGFβ2)/fibroblast growth factor 2 (FGF2), LIF regulates nephrogenesis. It facilitates tubular regeneration following acute kidney failure. It plays a crucial role in neuroprotective pathways induced by preconditioning-induced oxidative stress.[1] LIF is capable of maintaining the totipotency of embryonic stem cells.[3] It is also involved in controlling the differentiation of embryonic stem cells, hematopoietic and neuronal cells.[2]

Physical form

Lyophilized with no additives

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0817AFC32
0216AFC32
0107S243
0515AFC32

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ESGRO® Recombinant Mouse LIF Protein ESGRO Leukemia Inhibitory Factor (LIF) supplement for mouse ES cell culture. Each vial contains 10^7 units/ml.

Sigma-Aldrich

ESG1107

ESGRO® Recombinant Mouse LIF Protein

Protective effects of leukemia inhibitory factor against oxidative stress during high glucose-induced apoptosis in podocytes.
Xu J et al
Cell Stress & Chaperones, 17(4) (2012)
J F Moreau et al.
Nature, 336(6200), 690-692 (1988-12-15)
Leukaemia inhibitory factor (LIF) is a cytokine that induces macrophage differentiation of the murine M1 myeloid leukaemia cell line. We have isolated a cDNA clone encoding a novel human haemopoietic growth factor, human interleukin for DA cells (HILDA) that supports
The gp130 receptor cytokine family: regulators of adipocyte development and function.
White UA and Stephens JM
Current Pharmaceutical Design, 17(4), 340-346 (2011)
Complex conserved organization of the mammalian leukemia inhibitory factor gene: regulated expression of intracellular and extracellular cytokines.
Haines BP et al
Journal of Immunology, 162(8), 4637-4646 (1999)
Xiaoying Wang et al.
PloS one, 9(8), e106110-e106110 (2014-08-29)
Sertoli cells constitute the structural framework in testis and provide an immune-privileged environment for germ cells. Induced pluripotent stem cells (iPS cells) resemble embryonic stem cells (ES cells) and are generated from somatic cells by expression of specific reprogramming transcription
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