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L5283

Sigma-Aldrich

Leukemia Inhibitory Factor human

LIF, recombinant, expressed in E. coli, 10 μg/ml, buffered aqueous solution (pH 7.4), suitable for cell culture

Synonym(s):

Human LIF, Human Leukemia Inhibitory Factor, hLIF, LIF, rLIF

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About This Item

MDL number:
MFCD00163950
UNSPSC Code:
12352202
NACRES:
NA.77

recombinant

expressed in E. coli

Quality Level

200

Assay

≥95% (SDS-PAGE)

form

buffered aqueous solution (pH 7.4)

potency

0.1-1.5 ng/mL ED50/EC50

quality

endotoxin tested

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

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Biochem/physiol Actions

Leukemia Inhibitory Factor (LIF) is a pleiotropic glycoprotein originally described to inhibit the proliferation of the murine myeloid leukemic cell line M1, while inducing differentiation into macrophages. Other activities were later identified to LIF, which is known by a variety of synonyms, including DIF, D-factor, DIA, DRF, CNDF, HILDA, HSF-III, and MLPLI. Human LIF exerts its actions through a receptor comprising a 190 kDa LIF-binding α-chain (130 kDa, mouse) and a 130 kDa signal-transducing β-chain (gp130), which is shared with CNTF, OSM, L-6 and IL-11. As such, it is a member of the gp130 family of the cytokine receptor superfamily. LIF receptors have been identified on several cells, including monocytes, liver, placenta and embryonic stem cells. Natural LIF is heavily glycosylated, showing an apparent molecular weight of 32 kDa to 62 kDa, depending on the source, but absence of glycosylation appears not to affect its biological activity. A single gene encodes LIF, which is secreted as a single chain glycoprotein containing 180 amino acids for human or mouse with a conserved disulfide bond. Human and mouse LIF share 78% sequence homology. Human LIF can activate mouse cells, but mouse LIF cannot activate human cells.

Physical form

Solution, 0.2 μm filtered, in phosphate buffered saline containing 0.02% TWEEN® 20.

Analysis Note

The proliferative activity of human LIF is measured in culture using the human leukemic cell line, TF-1.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Magda Tomala et al.
Protein expression and purification, 73(1), 51-57 (2010-04-13)
Leukemia inhibitory factor (LIF) is a polyfunctional cytokine with numerous regulatory effects in vivo and in vitro. In stem cell cultures it is the essential media supplement for the maintenance of pluripotency of embryonic and induced pluripotent stem cells. With
N M Gough et al.
Proceedings of the National Academy of Sciences of the United States of America, 85(8), 2623-2627 (1988-04-01)
A human homologue of the recently cloned murine leukemia-inhibitory factor (LIF) gene was isolated from a genomic library by using the murine cDNA as a hybridization probe. The nucleotide sequence of the human gene indicated that human LIF has 78%
Dumrongkiet Arthan et al.
Cancer letters, 297(1), 31-41 (2010-06-24)
Medullary thyroid carcinoma (MTC) is a multiple endocrine neoplasia type 2 syndrome caused by mutations in extracellular receptor or intracellular kinase domains of the RET proto-oncogene. Activation of the Ras/Raf/MEK/ERK pathway can lead to growth arrest by secreting leukemia inhibitory
Borhane Annabi et al.
Journal of neuroinflammation, 6, 8-8 (2009-03-11)
The CD133(+) stem cell population in recurrent gliomas is associated with clinical features such as therapy resistance, blood-brain barrier disruption and, hence, tumor infiltration. Screening of a large panel of glioma samples increasing histological grade demonstrated frequencies of CD133(+) cells
Young Mok Lee et al.
Biology of reproduction, 75(3), 380-386 (2006-05-26)
In this study, we proposed a testis-mediated germline chimera production system based on the transplantation of testicular cells directly into heterologous testes. The testicular cells of juvenile (4-wk-old) or adult (24-wk-old) Korean Ogol chickens with a recessive pigmentation inhibitory gene
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