高级基因编辑
靶向基因组编辑已迅速成为贯穿从早期发现到治疗应用整体研究流程的重要工具。通过让科学家能够选择性地破坏、恢复、抑制或激活基因表达，现代基因编辑方法使得对控制生物过程的遗传机制进行前所未有的探索成为可能。基因工程的应用范围包括从治疗发育障碍到开发抗病作物。
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CRISPR – 准确、高效的基因编辑
基因编辑指的是针对DNA序列进行的一种特定且靶向的改变，涉及到DNA的添加、去除或修饰。CRISPR-Cas系统（在微生物中进化为一种防御机制）是一大类基因编辑工具的基础，其使得从健康和诊断到农业和能源的进步成为可能。通过CRISPR，研究人员拥有了靶向某个特定基因、基因家族甚至筛选整个基因组的能力。
CRISPR基因编辑系统示意图展示了用于CRISPR的不同类型合成gRNA。
Sigma-Aldrich® Advanced Genomics可提供行业领先的CRISPR-Cas9蛋白选择，以满足您的个性化研究需求。可选择多种Cas9突变体（野生型、特异性增强型、nickase切口酶型、GFP融合型、催化失活型)及多种形式（如质粒、慢病毒、冻干蛋白粉）。
担心CRISPR不适合您的实验项目？连线咨询Sigma-Aldrich® Advanced Genomics专家。
CRISPRi和CRISPRa – 强大的基因抑制和激活
CRISPRi（CRISPR干扰）和CRISPRa（CRISPR激活）可在不改变潜在的DNA序列的情况下分别提供高效的基因沉默和激活。当用于大规模 LOF（功能丧失）和 GOF（功能获得）筛选中时，研究人员能够识别出其他方法经常遗漏的独特但功能相关的基因通路。
Sigma-Aldrich® Advanced Genomics提供了一整套用于基因敲减和过表达实验的优化CRISPRi和CRISPRa文库目前可提供CRISPRi和SAM CRISPRa混合的慢病毒文库，也可根据您的特定需求提供定制产品。
担心CRISPR不适合您的实验项目？连线咨询Sigma-Aldrich®Advanced Genomics专家。
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