CRISPR Essentials: MISSION^® CRISPR gRNAs, Cas9 and Related Products

Compare and contrast the features of a wide variety of guide RNA (gRNA) and Cas9 products for in vitro and in vivo CRISPR experiments. Select the best formats for your mammalian or plant applications: SygRNA^® synthetic gRNA, plasmid DNA, lentiviral particles, and proteins.

• CRISPR Formats
• Cas9 Products
• CRISPR Controls
• Pricing Discounts
• Vectors

CRISPR Formats

SygRNA^® Synthetic gRNA

Product	Description	Specifications Available	Product Availability
Guaranteed Predesigned Synthetic CRISPR RNA	Synthetic CRISPR RNA with guaranteed activity. Optimized for knockout applications in the human and mouse genomes	crRNA: SpCas9 or FnCas9 species Desalt or HPLC purification Stabilizing modifications available 2 nmol or 5 nmol sgRNA: SpCas9 species HPLC purification Stabilizing modifications available 3 nmol	crRNA: Standard purification: Ships in 3-5 business days HPLC purification and modified HPLC purified: Ships in 5-7 business days sgRNA: ships in 7-10 business days
Custom Synthetic CRISPR RNA	Enter your own sequences or search 20+ genomes on the CRISPR Design Tool. All custom sequence gRNAs are guaranteed to work!	crRNA: SpCas9 or FnCas9 species Desalt or HPLC purification Stabilizing modifications available 2 nmol or 5 nmol sgRNA: SpCas9 species HPLC purification Stabilizing modifications available 3 nmol	crRNA: Standard purification: Ships in 3-5 business days HPLC purification and modified HPLC purified: Ships in 5-7 business days sgRNA: ships in 7-10 business days
TRACRRNA05N	SygRNA® SpCas9 tracrRNA	5 nmol	Off the Shelf
TRACRRNAMOD	SygRNA® SpCas9 Modified tracrRNA (pair with modified crRNA and SpCas9)	5 nmol	Off the Shelf
FNCAS9TRACR	SygRNA® FnCas9 tracrRNA (pair with unmodified crRNA and FnCas9)	5 nmol	Off the Shelf
Modified§ FNCAS9TRACR	SygRNA® Modified FnCas9 tracrRNA (pair with modified crRNA and FnCas9)	Request modified FntracrRNA	Please Inquire

SygRNA^® synthetic CRISPR two-part system requires both crRNA and tracrRNA to complex with Cas9. When ordering sgRNA or crRNA:tracrRNA, be sure to select the species that matches your Cas9 source.

View our protocols to learn more about SygRNA^® synthetic CRISPR.

For Sanger QuickPick™ Knockout gRNAs or custom gRNAs

No. of Clones Ordered	Techware	Volume	Product Availability
1-24	Individually labeled vials	500 µL	Ships in 2-3 days
25+	96 well plates	120 µL	Ships in 3-10 business days

For Sanger QuickPick™ Knockout gRNAs or custom gRNAs

Quantity of DNA ordered	Concentration	Techware	Volume	Product Availability– Sanger QuickPick™ Knockout Clones	Product Availability– Custom CRISPR Clones
1 µg	20 ng/µL	1-24 clones: individually labeled vials 25+ clones: 96 well plates	50 µL	Ships in 3 weeks; additional time for clone quantities > 100	Ships in 4 weeks; additional time for clone quantities > 100
10 µg 25 µg 100 µg	Concentration will be reported with product	individually labeled vials	Varies depending on concentration	Ships in 3-4 weeks; additional time for clone quantities > 25	Ships in 4-5 weeks; additional time for clone quantities > 25

For Sanger QuickPick™ Knockout gRNAs or custom gRNAs

Volume	Titer
	106	107	108	109
100 µL	N/A	N/A	2 x 50 µL 4 x 25 µL	5 x 20 µL
200 µL	1 x 200 µL 2 x 100 µL 4 x 50 µL 8 x 25 µL	1 x 200 µL 2 x 100 µL 4 x 50 µL 8 x 25 µL	4 x 50 µL 8 x 25 µL	10 x 20 µL
1 mL	5 x 200 µL 10 x 100 µL 20 x 50 µL 40 x 25 µL	5 x 200 µL 10 x 100 µL 20 x 50 µL 40 x 25 µL	20 x 50 µL 40 x 25 µL	50 x 20 µL
2 mL	10 x 200 µL 20 x 100 µL 40 x 50 µL 80 x 25 µL	10 x 200 µL 20 x 100 µL 40 x 50 µL 80 x 25 µL	40 x 50 µL 80 x 25 µL	100 x 20 µL
5 mL	25 x 200 µL 50 x 100 µL 100 x 50 µL 200 x 25 µL	25 x 200 µL 50 x 100 µL 100 x 50 µL 200 x 25 µL	100 x 50 µL 200 x 25 µL	N/A

Sanger QuickPick™ Knockout gRNA Lentivirus Manufacturing Timelines

Please allow for additional shipping time

Volume	Titer
	106	107	108	109
100 µL	N/A	N/A	5 weeks	5 weeks
200 µL	4 weeks	4 weeks	5 weeks	5 weeks
1 mL	5 weeks	5 weeks	5 weeks	5 weeks
2 mL	5 weeks	5 weeks	5 weeks	5 weeks
5 mL	5 weeks	5 weeks	5 weeks	N/A

CRISPR gRNA Lentivirus Manufacturing Timelines

Please allow for additional shipping time

Volume	Titer
	106	107	108	109
100 µL	N/A	N/A	6 weeks	6 weeks
200 µL	5 weeks	5 weeks	6 weeks	6 weeks
1 mL	6 weeks	6 weeks	6 weeks	6 weeks
2 mL	6 weeks	6 weeks	6 weeks	6 weeks
5 mL	6 weeks	6 weeks	6 weeks	N/A

Cas9 Products

Click on the product number to order and see additional product details

Cas9 Proteins

Product No.	Product	Description/Application
CAS9GFPPRO	Wild-type SpCas9-EGFP Fusion – NEW!	Original Wild-Type Cas9 fused to enhanced green fluorescent protein. Ideal for flow cytometry applications and visualization of transfected RNP complexes. Highly efficient
ECAS9GFPPR	Enhanced specificity SpCas9-EGFP Fusion – NEW!	Cas9 variant with Improved specificity fused to enhanced green fluorescent protein. Ideal for flow cytometry applications, fluorescence microscopy and for experiments that require high accuracy
CAS9PROT	Wild-type SpCas9	Original , wild-type Cas9; simple and economical; efficient in a broad range of gene editing applications
ESPCAS9PRO	Enhanced specificity SpCas9	Cas9 variant with improved specificity ideal for experiments that require high accuracy
CAS9D10APR	Cas9D10A Nickase	A two-gRNA system with improved specificity and decreased off-target potential for specialized genome-editing applications
DCAS9PROT	dCas9-3XFLAG™ Biotin	Catalytically inactive SpCas9 protein containing a 3XFLAGTM tag and Biotin; ideal for DNA detection and isolation and is a component of the proxy-CRISPR method
FNCAS9PROT	FnCas9 Protein	Ideal for synthetic biology or cell-free applications

Cas9 Plasmids and Lentivirus

Product No.	Description	Format	Selection Method
CAS9P	SpCas9 plasmid	Plasmid DNA
CAS9GFPP	SpCas9 GFP Plasmid	Plasmid DNA	Fluorophore (GFP)
CAS9RFPP	SpCas9 RFP Plasmid	Plasmid DNA	Fluorophore (RFP)
CAS9GEMP	SpCas9 GFP Geminin Plasmid	Plasmid DNA	Fluorophore (GFP)
CAS9D10AP	SpCas9-D10A Nickase Plasmid	Plasmid DNA
CAS9D10AGFPP	SpCas9-D10A Nickase GFP Plasmid	Plasmid DNA	Fluorophore (GFP)
CAS9D10ARFPP	SpCas9-D10A Nickase RFP Plasmid	Plasmid DNA	Fluorophore (RFP)
DCAS9P	SpdCas9 Plasmid	Plasmid DNA
DCAS9P300	SpdCas9-p300 Activator Plasmid	Plasmid DNA	Fluorophore (GFP)
DCAS9P300RFP	SpdCas9-p300 RFP Activator Expression Plasmid	Plasmid DNA	Fluorophore (RFP)
FNCAS9P	FnCas9 Plasmid	Plasmid DNA
CAS9MRNA	SpCas9 mRNA	mRNA
CAS9D10AMRNA	SpCas9-D10A Nickase mRNA	mRNA
CAS9BST	SpCas9 Blasticidin Lenti Plasmid	Plasmid DNA	Blasticidin
CAS9HYGROVP	SpCas9 Hygromycin Lenti Plasmid	Plasmid DNA	Hygromycin
CAS9NEO	SpCas9 Neomycin Lenti Plasmid	Plasmid DNA	Neomycin
CAS9ZEOVP	SpCas9 Zeo Lenti Plasmid	Plasmid DNA	Zeo
CAS9GFPVP	SpCas9 GFP Lenti Plasmid	Plasmid DNA	Fluorophore (GFP)
CAS9RFPVP	SpCas9 RFP Lenti Plasmid	Plasmid DNA	Fluorophore (RFP)
ESPCAS9BST	High Fidelity Sp Cas9 Blasticidin Lenti Plasmid	Plasmid DNA	Blasticidin
LVCAS9BST	SpCas9 Blasticidin Lenti Particles	Lentivirus	Blasticidin
LVCAS9NEO	SpCas9 Neomycin Lenti Particles	Lentivirus	Neomycin
CAS9BAC1P	SpCas9 Lambda Red Homologous Recombination Plasmid for E.coli	Plasmid DNA	Ampicillin

SAM CRISPR Activator Cas9 Products

Product No.	Description	Compatible gRNA vector	System	Format	Selection Method	Comment
SAMHELPERV	SAM CRISPRa Helper Construct Kit Lentiviral Transduction Particles	LV06 & LV07	Lentiviral	Lentivirus	Blasticidin & Hygromycin	Order compatible gRNA separately Kit contains 2 components: dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1 MS2-P65-HSF1-Hygromycin SAM CRISPRa Helper Construct 2
SAMHELPERP	SAM CRISPRa Helper Construct Kit Plasmid DNA	LV06 & LV07	Lentiviral	Plasmid DNA	Blasticidin & Hygromycin	Order compatible gRNA separately Kit contains 2 components: dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1 MS2-P65-HSF1-Hygromycin SAM CRISPRa Helper Construct 2
SAMVP64BSTV	dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1 Lentiviral Transduction Particles	LV06 & LV07	Lentiviral	Lentivirus	Blasticidin	Order compatible gRNA separately Requires MS2-P65-HSF1-Hygromycin SAM CRISPRa Helper Construct 2
SAMVP64BST	dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1 Plasmid DNA	LV06 & LV07	Lentiviral	Plasmid DNA	Blasticidin	Order compatible gRNA separately Requires MS2-P65-HSF1-Hygromycin SAM CRISPRa Helper Construct 2
SAMMS2HYGV	MS2-P65-HSF1-Hygromycin SAM CRISPRa Helper Construct 2 Lentiviral Transduction Particles	LV06 & LV07	Lentiviral	Lentivirus	Hygromycin	Order compatible gRNA separately Requires dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1
SAMMS2HYG	MS2-P65-HSF1-Hygromycin SAM CRISPRa Helper Construct 2 Plasmid DNA	LV06 & LV07	Lentiviral	Plasmid DNA	Hygromycin	Order compatible gRNA separately Requires dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1

CRISPR Inhibition Cas9 Products & Controls

Product No.	Description	Compatible gRNA Vector	System	Format	Selection Method	Comment
CRISPRICON	CRISPRi Control & Effector Kit	LV17	Lentiviral	Lentivirus	Puromycin & BFP	KRAB-dCas9 - Blasticidin CRISPRi Construct Lentiviral Transduction Particles CRISPRi RAB1A Puromycin Control Transduction Particles CRISPRi Nontargeting Control Puromycin Transduction Particles
CRISPRI10X	10X CRISPRi Feature Barcode Optimization Kit	LV13 LV14 LV15 LV16	Lentiviral	Lentivirus	Puromycin & BFP	RAB1A And Nontargeting Control With 10x Capture Sequence 1 Stem-Loop Position RAB1A And Nontargeting Control With 10x Capture Sequence 1 3 Prime Position RAB1A And Nontargeting Control With 10x Capture Sequence 2 Stem-Loop Position RAB1A And Nontargeting Control With 10x Capture Sequence 2 3 Prime Position

Vectors

Mammalian System Vectors

Vector No.	Description	Order Clones	Cas9/gRNA Content	Format Availability	Other Features
					Fluorophore	Cas9 type
p01	U6-gRNA:CMV-Cas9-2A-tGFP	p01 clones	Cas9+gRNA	Plasmid DNA	GFP	S. pyogenes
p02	U6-gRNA:CMV-Cas9-2A-tRFP	p02 clones	Cas9+gRNA	Plasmid DNA	RFP	S. pyogenes
p04	U6-gRNA:CMV-eCas9-2a-tGFP	p04 clones	Cas9+gRNA	Plasmid DNA	GFP	enhanced specificity
p03	U6-gRNA	p03 clones	gRNA only	Plasmid DNA

Lentiviral Vectors

Vector No.	Description	Order Clones	Cas9/gRNA Content	Format Availability	Selection Method
LV01	U6-gRNA:ef1a-puro-2A-Cas9-2A-tGFP	LV01 clones	Cas9+gRNA	Plasmid DNA & Lentivirus	Fluorophore (GFP) & Puromycin
LV05	U6-gRNA:EF1a-Cas9+FLAG-2A-Puro	LV05 clones	Cas9+gRNA	Plasmid & Lentivirus	Puromycin
LV06	CRISPRa SAM U6-gRNA:EF1a-Puro	LV06 clones	CRISPR activator gRNA	Plasmid & Lentivirus	Puromycin
LV07	CRISPRa SAM U6-gRNA:EF1a-Zeo	LV07 clones	CRISPR activator gRNA	Plasmid & Lentivirus	Zeo
LV04	U6-gRNA:hPGK-puro-2A-tBFP	Sanger QP KO clones	gRNA only	Plasmid DNA & Lentivirus	Fluorophore (BFP) & Puromycin
LV03	U6-gRNA:ef1a-tGFP	LV03 clones	gRNA only	Plasmid DNA & Lentivirus	Fluorophore (GFP)
LV02	U6-gRNA:ef1a-puro	LV02 clones	gRNA only	Plasmid DNA & Lentivirus	Puromycin

Plant Vectors

Vector No.	Description	Order Clones	Cas9/gRNA Content	Format Availability	Species	Plasmid Type	Selection
p63	(dicot)U6-gRNA:CMV-Cas9-beta glucuronidase (Agrobacterium plasmid)	p63 clones	Cas9+ gRNA	Plasmid DNA	Dicot	Agro-bacterium	Beta Glucuron- idase
p55	(dicot)U6-gRNA:CMV-Cas9-bar (Agrobacterium plasmid)	p55 clones	Cas9+ gRNA	Plasmid DNA	Dicot	Agro-bacterium	Biala- phos
p53	(dicot)U6-gRNA:CMV-Cas9-hyg (Agrobacterium plasmid)	p53 clones	Cas9+ gRNA	Plasmid DNA	Dicot	Agro-bacterium	Hygro- mycin
p54	(dicot)U6-gRNA:CMV-Cas9-neo (Agrobacterium plasmid)	p54 clones	Cas9+ gRNA	Plasmid DNA	Dicot	Agro-bacterium	Neo- mycin
p61	(dicot)U6-gRNA:CMV-Cas9-bar (bioballistic plasmid)	p61 clones	Cas9+ gRNA	Plasmid DNA	Dicot	Bio-Ballistic	Biala- phos
p59	(dicot)U6-gRNA:CMV-Cas9-hyg (bioballistic plasmid)	p59 clones	Cas9+ gRNA	Plasmid DNA	Dicot	Bio-Ballistic	Hygro- mycin
p60	(dicot)U6-gRNA:CMV-Cas9-neo (bioballistic plasmid)	p60 clones	Cas9+ gRNA	Plasmid DNA	Dicot	Bio-Ballistic	Neo- mycin
p62	(monocot)U6-gRNA:CMV-Cas9-beta glucuronidase (Agrobacterium plasmid)	p62 clones	Cas9+ gRNA	Plasmid DNA	Monocot	Agro-bacterium	Beta Glucuron- idase
p52	(monocot)U6-gRNA:CMV-Cas9-bar (Agrobacterium plasmid)	p52 clones	Cas9+ gRNA	Plasmid DNA	Monocot	Agro-bacterium	Biala- phos
p50	(monocot) U6-gRNA:CMV-Cas9-hyg (Agrobacterium plasmid)	p50 clones	Cas9+ gRNA	Plasmid DNA	Monocot	Agro-bacterium	Hygro- mycin
p51	(monocot)U6-gRNA:CMV-Cas9-neo (Agrobacterium plasmid)	p51 clones	Cas9+ gRNA	Plasmid DNA	Monocot	Agro-bacterium	Neo- mycin
p58	(monocot)U6-gRNA:CMV-Cas9-bar (bioballistic plasmid)	p58 clones	Cas9+ gRNA	Plasmid DNA	Monocot	Bio-Ballistic	Biala- phos
p56	(monocot)U6-gRNA:CMV-Cas9-hyg (bioballistic plasmid)	p56 clones	Cas9+ gRNA	Plasmid DNA	Monocot	Bio-Ballistic	Hygro- mycin
p57	(monocot)U6-gRNA:CMV-Cas9-neo (bioballistic plasmid)	p57 clones	Cas9+ gRNA	Plasmid DNA	Monocot	Bio-Ballistic	Neo- mycin

Bacterial Editors

Vector No.	Description	Order Clones	Cas9/gRNA Content	Format Availability	Selection
p10	(bacteria) J23119-gRNA-amp	p10 clones	gRNA only	Plasmid DNA	Ampicillin

CRISPR Controls

Click on the product number to order and see additional product details

Product No.	Description	Vector	Control Type	System	Format	gRNA Type	Selection Method
CRISPR06	CRISPR Universal Non-target Negative Control 1, Cas9+gRNA Plasmid	p01	Neg.	Mammalian	Plasmid DNA	Cas9+gRNA	Fluorophore (GFP)
CRISPR07	CRISPR Universal Non-target Negative Control 2, Cas9+gRNA Plasmid	p01	Neg.	Mammalian	Plasmid DNA	Cas9+gRNA	Fluorophore (GFP)
CRISPR08	CRISPR Universal Non-target Negative Control 3, Cas9+gRNA Plasmid	p01	Neg.	Mammalian	Plasmid DNA	Cas9+gRNA	Fluorophore (GFP)
CRISPR17	CRISPR Activator Human Oct4 Positive Control Plasmid, gRNA Only Expression Plasmid	p03	Pos.	Mammalian	Plasmid DNA	gRNA only
CRISPR12	CRISPR-Lenti Universal Non-Target Negative Control 1, Cas9+gRNA Lenti Plasmid (vector)	LV01	Neg.	Lentiviral	Plasmid DNA	Cas9+gRNA	Fluorophore (GFP) & Puromycin
CRISPR18	CRISPR-Lenti Universal Non-target Negative Control 1, gRNA Only Lenti Plasmid (Sanger vector)	LV04	Neg.	Lentiviral	Plasmid DNA	gRNA only	Fluorophore (BFP) & Puromycin
CRISPR19	CRISPR-Lenti Universal Non-target Negative Control 2, gRNA Only Lenti Plasmid (Sanger vector)	LV04	Neg.	Lentiviral	Plasmid DNA	gRNA only	Fluorophore (BFP) & Puromycin
CRISPR20	CRISPR-Lenti Universal Non-target Negative Control 3, gRNA Only Lenti Plasmid (Sanger vector)	LV04	Neg.	Lentiviral	Plasmid DNA	gRNA only	Fluorophore (BFP) & Puromycin
CRISPR12V	CRISPR-Lenti Universal Non-Target Negative Control 1, Cas9+gRNA Lentiviral Particles (vector)	LV01	Neg.	Lentiviral	Lentivirus	Cas9+gRNA	Fluorophore (GFP) & Puromycin
CRISPR12H	CRISPR-Lenti Universal Non-Target Negative Control 1, Cas9+gRNA Lentiviral Particles, High Titer (vector)	LV01	Neg.	Lentiviral	Lentivirus	Cas9+gRNA	Fluorophore (GFP) & Puromycin
CRISPR15H	Gecko2-Lenti Universal Non-Target Negative Control 1, gRNA Only High Titer Lentiviral Particles (Gecko2 vector)	LV02	Neg.	Lentiviral	Lentivirus	gRNA only	Puromycin
CRISPR18V	CRISPR-Lenti Universal Non-target Negative Control 1, gRNA Only Lentiviral Particles (Sanger vector)	LV04	Neg.	Lentiviral	Lentivirus	gRNA only	Fluorophore (BFP) & Puromycin
CRISPR19V	CRISPR-Lenti Universal Non-target Negative Control 2, gRNA Only Lentiviral Particles (Sanger vector)	LV04	Neg.	Lentiviral	Lentivirus	gRNA only	Fluorophore (BFP) & Puromycin
CRISPR20V	CRISPR-Lenti Universal Non-target Negative Control 3, gRNA Only Lentiviral Particles (Sanger vector)	LV04	Neg.	Lentiviral	Lentivirus	gRNA only	Fluorophore (BFP) & Puromycin
CRISPR01	CRISPR Human EMX1 Positive Control Plasmid, gRNA Only Expression Plasmid, includes Cas9 Expression Plasmid (vector)	p03	Pos.	Mammalian	Plasmid DNA	gRNA only
CRISPR02	CRISPR Nickase Human EMX1 Positive Control Plasmids (gRNA Only Expression Plasmids, Includes Cas9-D10A Plasmid)	p03	Pos.	Mammalian	Plasmid DNA	gRNA only
CRISPR11	CRISPR-Lenti Human EMX1 Positive Control, Cas9+gRNA Lenti Plasmid (vector)	LV01	Pos.	Lentiviral	Plasmid DNA	Cas9+gRNA	Fluorophore (GFP) & Puromycin
CRISPR13	CRISPR-Lenti Human HPRT1 Positive Control, Cas9+gRNA Lenti Plasmid (vector)	LV01	Pos.	Lentiviral	Plasmid DNA	Cas9+gRNA	Fluorophore (GFP) & Puromycin
CRISPR21	CRISPR-Lenti Human HPRT1 Positive Control, gRNA Only Lenti Plasmid (Sanger vector)	LV04	Pos.	Lentiviral	Plasmid DNA	gRNA only	Fluorophore (BFP) & Puromycin
CRISPR11V	CRISPR-Lenti Human EMX1 Positive Control, Cas9+gRNA Lentiviral Particles (vector)	LV01	Pos.	Lentiviral	Lentivirus	Cas9+gRNA	Fluorophore (GFP) & Puromycin
CRISPR13V	CRISPR-Lenti Human HPRT1 Positive Control, Cas9+gRNA Lentiviral Particles (vector)	LV01	Pos.	Lentiviral	Lentivirus	Cas9+gRNA	Fluorophore (GFP) & Puromycin
CRISPR13H	CRISPR-Lenti Human HPRT1 Positive Control, Cas9+gRNA Lentiviral Particles, High Titer (vector)	LV01	Pos.	Lentiviral	Lentivirus	Cas9+gRNA	Fluorophore (GFP) & Puromycin
CRISPR16H	Gecko2-Lenti Human HPRT1 Positive Control, gRNA Only High Titer Lentiviral Particles (Gecko2 vector)	LV02	Pos.	Lentiviral	Lentivirus	gRNA only	Puromycin
CRISPR21V	CRISPR-Lenti Human HPRT1 Positive Control, gRNA Only Lentiviral Particles (Sanger vector)	LV04	Pos.	Lentiviral	Lentivirus	gRNA only	Fluorophore (BFP) + Puromycin
CRISPRPL01	CRISPR GUS GAPDH Reporter Control for Monocots	p62	Pos.	Plant	Plasmid DNA	Cas9+gRNA	Beta Glucuronidase
CRISPRPL02	CRISPR GUS GAPDH Reporter Control for Dicots	p63	Pos.	Plant	Plasmid DNA	Cas9+gRNA	Beta Glucuronidase

Volume Price Discounts

Product	Standard Offering	Quantities 25 - 100	Quantities 101 - 1000
Sanger QuickPick™ KO Bacteria Glycerol Stocks	500 µL or 120 µL of bacteria glycerol stock	20% discount	40% discount
Sanger QuickPick™ KO Plasmid DNA	1 µg Plasmid DNA at 20 ng/µL	20% discount*	40% discount*
Sanger QuickPick™ KO Lentivirus	1 x 200 µL lentivirus, minimum titer 1e6 TU/mL	20% discount**	40% discount**
Custom CRISPR Plasmid DNA	1 µg Plasmid DNA at 20 ng/µL	15% discount*	30% discount*
Custom CRISPR Lentivirus	1 x 200 µL lentivirus, minimum titer 1e6 TU/mL	15% discount**	30% discount**
Custom CRISPR IVT RNA	20 µL IVT RNA at 200 ng/µL	15% discount	30% discount

Bacteria Glycerol Stocks: provided in Terrific Broth supplemented with carbenicllin (100 µg/mL) and 10% glycerol

Plasmid DNA: provided in 1xTris-EDTA

Lentivirus: provided in Dulbecco's Modified Eagle's Medium supplemented with 10% Fetal Bovine Serum and 1% Penicillin/Streptomycin

For all custom inquiries or additional information Contact Us.

Vector Maps and Sequences

Click on the images below for a larger view.

p01 Vector Download Fully Annotated p01 Map Download p01 FASTA Sequence	p02 Vector Download Fully Annotated p02 Map Download p02 FASTA Sequence	p03 Vector Download Fully Annotated p03 Map Download p03 FASTA Sequence
p04 Vector Download Fully Annotated p04 Map Download p04 FASTA Sequence	p05 Vector Download Fully Annotated p05 Map Download p05 FASTA Sequence	p10 Vector Download Fully Annotated p10 Map Download p10 FASTA Sequence
p50 Vector Download Fully Annotated p50 Map Download p50 FASTA Sequence	p51 Vector Download Fully Annotated p51 Map Download p51 FASTA Sequence	p52 Vector Download Fully Annotated p52 Map Download p52 FASTA Sequence
p53 Vector Download Fully Annotated p53 Map Download p53 FASTA Sequence	p54 Vector Download Fully Annotated p54 Map Download p54 FASTA Sequence	p55 Vector Download Fully Annotated p55 Map Download p5 FASTA Sequence
p56 Vector Download Fully Annotated p56 Map Download p56 FASTA Sequence	p57 Vector Download Fully Annotated p57 Map Download p57 FASTA Sequence	p58 Vector Download Fully Annotated p58 Map Download p58 FASTA Sequence
p59 Vector Download Fully Annotated p59 Map Download p59 FASTA Sequence	p60 Vector Download Fully Annotated p60 Map Download p60 FASTA Sequence	p61 Vector Download Fully Annotated p61 Map Download p61 FASTA Sequence
p62 Vector Download Fully Annotated p62 Map Download p62 FASTA Sequence	p63 Vector Download Fully Annotated p63 Map Download p01 FASTA Sequence	LV01 Vector Download Fully Annotated LV01 Map Download LV01 FASTA Sequence
LV02 Vector Download Fully Annotated LV02 Map Download LV02 FASTA Sequence	LV03 Vector Download Fully Annotated LV03 Map Download LV03 FASTA Sequence	LV04 Vector Download Fully Annotated LV04 Map Download LV04 FASTA Sequence
LV05 Vector Download Fully Annotated LV05 Map Download LV05 FASTA Sequence	LV06 Vector Download Fully Annotated LV06 Map Download LV06 FASTA Sequence	LV07 Vector Download Fully Annotated LV07 Map Download LV07 FASTA Sequence
LV17 Vector	KRAB-dCas9 Vector

Product Guarantee

We are so confident in the performance of our SygRNA^® products, that we fully guarantee the quality and performance of any gRNA we produce, including custom sequences. If your crRNA or sgRNA do not yield detectable cleavage at the intended target site, we will provide you a one-time replacement, free of charge.

To qualify for this guarantee, please send an image or sequencing data from a single experiment demonstrating detectable cleavage using one of our positive controls, side-by-side with the negative results from your SygRNA^® gRNA. To receive your replacement, simply email oligotechserv@milliporesigma.com and include sample data from a representative experiment (T7E1, TIDE, or NGS)