HPLC Analysis of Maté Leaves on Ascentis® Express C18
CONDITIONS
sample/matrix
UAE (Ultrasonic Extraction) maté extract (dried and minced leaves (7.5 g) from maté were extracted with 225 mL of methanol in an ultrasonic bath, thermostated at 75 ± 0.5 °C (potency: 90 W; frequency: 40 kHz))
column
Ascentis Express C18, 150 x 4.6 mm I.D., 2.7 μm particles (53829-U)
mobile phase
[A] water, pH 3 with formic acid; [B] acetonitrile, pH 3 with formic acid
gradient
0 to 5% B in 20 min; 5 to 40% B in 30 min
flow rate
1 mL/min
column temp.
25 °C
detector
PDA: range 215- 420 nm (8 μL detector cell volume, cell temp. 40 °C, sampling rate 12.5 Hz, time constant 0.025 s)
injection
10 μL
说明
法律資訊
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany