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HPLC Analysis of Aminopterin and Related Compounds on Ascentis® Express C18

HPLC Analysis of Aminopterin and Related Compounds on Ascentis® Express C18 application for HPLC

材料

分析柱

产品编号
说明
价格

Ascentis® Express C18, 2.7 μm HPLC 色谱柱

2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

標準品

产品编号
说明
价格

叶酸

≥97%

甲氨蝶呤

meets USP testing specifications

CONDITIONS

column

Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 μm particles (53829-U)

mobile phase

[A] 10 mM ammonium formate, pH 3.0; [B] acetonitrile; (90:10, A:B)

flow rate

1.0 mL/min

column temp.

35 °C

detector

UV, 280 nm

injection

5 μL

说明

分析報告

This application demonstrates the suitability of the Ascentis C18 for the analysis of aminopterin, folic acid and methotrexate. Structures along with the optimized chromatogram obtained on the Ascentis Express C18 are presented

法律資訊

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany