跳转至内容
Merck
  • System identification of signaling dependent gene expression with different time-scale data.

System identification of signaling dependent gene expression with different time-scale data.

PLoS computational biology (2017-12-28)
Takaho Tsuchiya, Masashi Fujii, Naoki Matsuda, Katsuyuki Kunida, Shinsuke Uda, Hiroyuki Kubota, Katsumi Konishi, Shinya Kuroda
摘要

Cells decode information of signaling activation at a scale of tens of minutes by downstream gene expression with a scale of hours to days, leading to cell fate decisions such as cell differentiation. However, no system identification method with such different time scales exists. Here we used compressed sensing technology and developed a system identification method using data of different time scales by recovering signals of missing time points. We measured phosphorylation of ERK and CREB, immediate early gene expression products, and mRNAs of decoder genes for neurite elongation in PC12 cell differentiation and performed system identification, revealing the input-output relationships between signaling and gene expression with sensitivity such as graded or switch-like response and with time delay and gain, representing signal transfer efficiency. We predicted and validated the identified system using pharmacological perturbation. Thus, we provide a versatile method for system identification using data with different time scales.

材料
货号
品牌
产品描述

Sigma-Aldrich
杜氏改良 Eagle 培养基-低葡萄糖, With 1000 mg/L glucose, L-glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
SKU
包装规格
是否有货
价格
数量
Sigma-Aldrich
垂体腺苷酸环化酶激活多肽-38
SKU
包装规格
是否有货
价格
数量