Oncogenic PIK3CA induces centrosome amplification and tolerance to genome doubling.

Oncogenic PIK3CA induces centrosome amplification and tolerance to genome doubling.

Nature communications (2017-11-25)

Inma M Berenjeno, Roberto Piñeiro, Sandra D Castillo, Wayne Pearce, Nicholas McGranahan, Sally M Dewhurst, Valerie Meniel, Nicolai J Birkbak, Evelyn Lau, Laurent Sansregret, Daniele Morelli, Nnennaya Kanu, Shankar Srinivas, Mariona Graupera, Victoria E R Parker, Karen G Montgomery, Larissa S Moniz, Cheryl L Scudamore, Wayne A Phillips, Robert K Semple, Alan Clarke, Charles Swanton, Bart Vanhaesebroeck

PMID29170395

摘要

Mutations in PIK3CA are very frequent in cancer and lead to sustained PI3K pathway activation. The impact of acute expression of mutant PIK3CA during early stages of malignancy is unknown. Using a mouse model to activate the Pik3ca

材料

货号

品牌

产品描述

P8920

Sigma-Aldrich

多聚-L-赖氨酸溶液, 0.1 % (w/v) in H₂O

D9663

Sigma-Aldrich

驴血清

G9023

Sigma-Aldrich

山羊血清

H7904

Sigma-Aldrich

(Z)-4-羟三苯氧胺, ≥98% Z isomer

05-636

Sigma-Aldrich

抗磷酸组蛋白H2A.X（Ser139）抗体，克隆JBW301, clone JBW301, Upstate^®, from mouse

A1978

Sigma-Aldrich

抗 β-肌动蛋白抗体，小鼠单克隆, clone AC-15, purified from hybridoma cell culture

M1404

Sigma-Aldrich

诺考达唑, ≥99% (TLC), powder

CS0030

Sigma-Aldrich

衰老细胞组织化学染色试剂盒, sufficient for 100 tests

T6074

Sigma-Aldrich

抗-α微管蛋白抗体，小鼠单克隆抗体, clone B-5-1-2, purified from hybridoma cell culture

R5503

Sigma-Aldrich

核糖核酸酶A 来源于牛胰腺, Type I-AS, 50-100 Kunitz units/mg protein

X100

Sigma-Aldrich

Triton^™ X-100, laboratory grade

T4424

Sigma-Aldrich

胰蛋白酶溶液来源于猪胰腺, 1 ×, sterile-filtered, BioReagent, suitable for cell culture, 2.5 g porcine trypsin per liter in Hanks′ Balanced Salt Solution with phenol red

A7906

Sigma-Aldrich

牛血清白蛋白来源于牛血清, heat shock fraction, pH 7, ≥98%

A5044

Sigma-Aldrich

单克隆抗 α-辅肌动蛋白小鼠抗, clone BM-75.2, ascites fluid

D1641

Sigma-Aldrich

二氢细胞松弛素 B