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用途
sufficient for 100 tests
品質等級
包裝
pkg of 1 kit
儲存條件
dry at room temperature
檢測方法
colorimetric
運輸包裝
dry ice
儲存溫度
−20°C
一般說明
衰老细胞组织化学染色试剂盒含有鉴定衰老细胞所需的所有试剂，测试基于pH 6下ß-半乳糖苷酶活性的组织化学染色。ß-衰老细胞中的半乳糖苷酶活性是可检测的，靜止細胞、永生细胞或肿瘤细胞则无法检测。
衰老细胞组织化学染色试剂盒含有鉴定衰老细胞所需的所有试剂，测试基于pH 6下β-半乳糖苷酶活性的组织化学染色。β-衰老细胞中的半乳糖苷酶活性是可检测的，靜止細胞、永生细胞或肿瘤细胞则无法检测。细胞衰老是一系列事件的进展，细胞借此由活跃分裂转为不分裂期。细胞衰老过程和老化相关。细胞分裂的减少几乎是不可逆的、完全的。除了丧失分裂能力外，细胞的形态学、形状和物理外观，以及它们的基因表达模式也发生变化。在该过程的结束时，细胞常常死亡，尽管细胞可能仍长期保持活力。
應用
衰老细胞组织化学染色试剂盒已被用于：
- 与衰老相关的β-半乳糖苷酶测试
- 测试人胰腺癌细胞
- 脂肪源性干细胞（ADSC）
- 脐血间充质/干细胞（CBMSC）中的细胞衰老
生化／生理作用
复制性衰老是一种生长停滞状态，主要表现为细胞失去分裂潜能，同时细胞形态、形状、生理特征和基因表达谱发生变化。
法律資訊
本产品根据美国专利号：第5491069和5795728号授权生产。
仅试剂盒组分
产品编号
说明
- X-gal solution 4 mL
- Staining Solution, 10X 15 mL
- Fixation Buffer 10x 15 mL
- Reagent B 1.5 mL
- Reagent C 1.5 mL
- Dulbecco's Phosphate Buffered Saline (PBS) 10x 60 mL
相關產品
訊號詞
Danger
危險分類
Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Muta. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
標靶器官
Respiratory system
安全危害
儲存類別代碼
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
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Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
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