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HPLC: suitable
測試參數
SPE tube/cartridge: Discovery DSC-Si, 1 gm/6 mL (52656-U)
condition: 6 mL hexane
sample addition: 0.12 g oil in 0.5 mL hexane
elution: 10 mL hexane-diethyl ether 87-13 (v/v)
eluate post-treatment: evaporation to dryness, reconstituted into 2 mL acetone
column: Discovery HS C18, 25 cm x 4.6 mm I.D., 5 μm particles (568523-U)
mobile phase: [A] acetone; [B] acetonitrile; (60:40, A:B)
flow rate: 1.5 mL/min
pressure: 914 psi (63 bar)
column temp.: 30 °C
detector: ELSD
injection: 5 μL
適合性
application for HPLC
應用
food and beverages
分析報告
Olive oil was pre-cleaned using Silica SPE, analyzed as 5% sample in acetone (w/v).
法律資訊
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
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