G005797

HPLC Analysis of Aspirin and Salicylic Acid on Ascentis^® Express RP-Amide

application for HPLC

属性

• 技術: HPLC: suitable
• 測試參數: column: Ascentis Express RP-Amide, 10 cm x 4.6 mm I.D., 2.7 μm particles (53929-U); mobile phase: [A] water with 0.1% TFA; [B] acetonitrile with 0.1% TFA; (65:35, A:B); flow rate: 1 mL/min; pressure: 2200 psi (152 bar); column temp.: 30 °C; detector: UV, 250 nm; injection: 5 μL; sample: 50 μg/mL in 75:25, water:acetonitrile
• 適合性: application for HPLC
• 應用: pharmaceutical (small molecule)

说明

分析報告

Salicylic acid is the primary metabolite of aspirin, and is likely responsible for its antiinflammatory properties via suppression of cyclooxygenase genes; it also is thought to suppress colonic carcinogenesis. Ascentis Express RP-Amide provides good retention and resolution at low pH by a combination of hydrophobic and H-bonding intereactions.

法律資訊

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

分析物

Acetylsalicylic acid, analytical standard
Salicylic acid, BioXtra, ≥99.0%