技術
HPLC: suitable
測試參數
column: Ascentis Express Phenyl-Hexyl, 10 cm x 2.1 mm I.D., 2.7 μm particles (53336-U)
mobile phase: [A] water; [B] acetonitrile
gradient: 20 to 100% B in 10 min; held at 100% B for 0.5 min
flow rate: 0.3 mL/min
pressure: 2321 psi (160 bar)
column temp.: 35 °C
detector: UV, 227 nm
injection: 5 μL
sample: 20 μg/mL in water: methanol (80:20)
適合性
application for HPLC
應用
vitamins, nutraceuticals, and natural products
分析報告
This application demonstrates the suitability of Ascentis Express Phenyl-Hexyl for the efficient separation of withania standards.
法律資訊
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
分析物
说明
Withanoside IV
Withanoside V
Withaferin A
12-Deoxywithastramonolide
Withanolide A
Withanone
Withanolide B
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