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Key Documents

G005619

LC/MS/MS Analysis of Carbendazim in Orange Juice (Spiked) on Ascentis® Express C18 after Supel QuE PSA/C18 QuEChERS Cleanup

application for HPLC, application for SPE

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About This Item

技術

HPLC: suitable
solid phase extraction (SPE): suitable

測試參數

sample preparation: Dispersive (QuEChERS)
sample/matrix: orange juice spiked with carbendazim at 1 ppb
extraction tube: Supel QuE Citrate (EN) Tube (55227-U)
extraction process: add 10 mL of orange juice with pulp to a 50 mL empty extraction tube (55248-U); add carbendazim at 1 ppb; add 10 mL acetonitrile; (shake for 1 minute; add contents of citrate extraction tube (55227-U); shake for 1 min; centrifuge at 3200 rpm for 5 minutes)
clean-up tube: Supel-Q PSA/C18 (55288-U)
clean-up process: transfer 0.7 mL of the acetonitrile layer to the PSA/C18 cleanup tube (55288-U); shake 1 minute; (centrifuge for 3 minutes at 3400 rpm; remove 0.25 mL of the supernatant; mix with 0.25 mL water for LC-MS analysis)
column: Ascentis Express C18, 5 cm x 2.1 mm I.D., 2.7 μm particles (53822-U)
mobile phase: [A] 10 mM ammonium acetate in water; [B] 10 mM ammonium acetate in methanol
gradient: 0-1 min: 30% B; 1.5-3.5 min: 100% B; 3.5-7 min: 30% B
flow rate: 0-1 min: 0.3 mL/min; 1.5-7 min: 0.5 mL/min
column temp.: 30 °C
detector: MS, ESI(+), MRM, m/z 192/160, 192/132
injection: 5 μL

適合性

application for HPLC
application for SPE

應用

food and beverages

分析報告

Simple and fast extraction and analytical method were developed for analysis of fungicide carbendazim in orange juice samples. QuEChERS extraction method was used with PSA/C18 cleanup. Ascentis Express C18 column chromatography was coupled to MS/MS detection with limits of detection at 0,1 ppb.

法律資訊

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Supel is a trademark of Sigma-Aldrich Co. LLC

分析物

说明

    Carbendazim, PESTANAL®, analytical standard

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