G005601

HPLC Analysis of Levothyroxine and Liothyronine on Ascentis^® Express ES-Cyano

application for HPLC

属性

• 技術: HPLC: suitable
• 測試參數: column: Ascentis Express ES-Cyano, 10 cm x 2.1 mm I.D., 2.7 μm particles (53491-U); mobile phase: [A] water with 0.05% phosphoric acid; [B] acetonitrile with 0.05% phophoric acid; (60:40, A:B); flow rate: 1.5 mL/min; pressure: 3270 psi (225 bar); column temp.: 30 °C; detector: UV, 225 nm; injection: 5 μL; sample: 20 μg/mL in mobile phase
• 適合性: application for HPLC
• 應用: clinical

说明

分析報告

This application demonstrates the suitabilty of Ascentis Express ES-Cyano for the analysis of Levothyroxine according to USP Monograph 3301 with Liothyronine as a reference standard.

法律資訊

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

分析物

3,3′,5-Triiodo-L-thyronine sodium salt, powder, BioReagent, suitable for cell culture
Levothyroxine sodium hydrate, PESTANAL^®, analytical standard