技術
HPLC: suitable
測試參數
column: TSKgel CM-STAT, 10 cm x 4.6 mm I.D., 7 μm particles (821966)
mobile phase: [A] 20 mM MES buffer, pH 6.0; [B] 0.5 M NaCl in buffer A, pH 6.0
gradient: 0% B (0 min), 30% B (15 min), 100% B (15 min),
0% B (17 min), 10% B (17 min), 10% B (21 min)
flow rate: 1 mL/min
column temp.: ambient
detector: UV, 280 nm
injection: 20 μL
sample: monoclonal antibodies (mAb A through E)
適合性
application for HPLC
應用
life science and biopharma
分析報告
A TSKgel CM-STAT weak cation exchange (WCX) column was applied to separate charge variants of several monoclonal antibodies. The typical analysis time on conventional 25 cm long WCX columns of about eighty minutes could be significantly reduced when separation was performed on a 10 cm TSKgel CM-STAT column, filled with 7 μm particles. The analysis profiles for five antibodies show that high resolution analysis can be obtained in about 20 minutes analysis time.
引用
From "Ion Exchange Chromatography for the Characterization of Biotherapeutics" in The Supelco Reporter, Vol. 29.3, page 20.
法律資訊
TSKgel is a registered trademark of Tosoh Corporation
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